VPS24 Polyclonal antibody

• Featured Product
• KD/KO Validated

VPS24 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15472-1-AP

Synonyms

hVps24, Chromatin-modifying protein 3, Chromatin modifying protein 3, CHMP3, CGI-149

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Validation Data Gallery

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  WB analysis of HEK-293 using 15472-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15472-1-AP (VPS24 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 15472-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15472-1-AP (VPS24 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15472-1-AP

  IP result of anti-VPS24 (IP:15472-1-AP, 4ug; Detection:15472-1-AP 1:2000) with HEK-293 cells lysate 1440 ug.

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  IHC staining of human heart using 15472-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15472-1-AP (VPS24 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 15472-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15472-1-AP (VPS24 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 15472-1-AP

  Immunofluorescent analysis of HepG2 cells, using VPS24 antibody 15472-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human kidney tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15472-1-AP targets VPS24 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS24 fusion protein Ag7776
• Full Name: vacuolar protein sorting 24 homolog (S. cerevisiae)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC004419
• Gene Symbol: VPS24
• Gene ID (NCBI): 51652
• RRID: AB_2217102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier
human	WB	J Cell Biol; ALIX- and ESCRT-III-dependent sorting of tetraspanins to exosomes.; Authors - Jorge Larios ; KD Validated
human	WB,IHC	Int J Oncol; CHMP3 promotes the progression of hepatocellular carcinoma by inhibiting caspase‑1‑dependent pyroptosis; Authors - Yuting Zheng ; KD Validated
human	WB	J Extracell Biol; RAB31 drives extracellular vesicle fusion and cancer-associated fibroblast formation leading to oxaliplatin resistance in colorectal cancer; Authors - Yu-Chan Chang