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CHMP2B Polyclonal antibody

CHMP2B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12527-1-AP

Synonyms

CHMP2.5, CHMP2B, chromatin modifying protein 2B, DMT1, hVps2 2, VPS2 2, VPS2B



Tested Applications

Positive WB detected inNIH/3T3 cells, human heart tissue, mouse brain tissue, human ileum tissue, human kidney tissue, human placenta tissue, human brain tissue
Positive IHC detected inmouse brain tissue, human brain tissue, human liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, PFA fixed cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12527-1-AP targets CHMP2B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHMP2B fusion protein Ag3222
Full Name chromatin modifying protein 2B
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC001553
Gene Symbol CHMP2B
Gene ID (NCBI) 25978
RRIDAB_10603358
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHMP2B, chromatin-modifying protein 2b, also named CHMP2.5, VPS2B, and VPS2 2, belongs to the chromatin-modifying protein / charged multivesicular body protein (CHMP) family. It is a component of the endosomal sorting complex required for transport III (ESCRT-III), which involves in endosomal and autophagic trafficking of proteins to lysosomes for degradation. Mutations of CHMP2B lead to C-terminal truncation or are replaced with mis-splicing C-termini and cause frontotemporal lobar degeneration (FTLD). In CHMP2B mutation patients, p62- and ubiquitin-positive, but TDP-43 and FUS negative neural inclusions are formed, which may be caused by impaired lysosomal degradation through the autophagy and endosome-lysosome pathways.

Protocols

Product Specific Protocols
WB protocol for CHMP2B antibody 12527-1-APDownload protocol
IHC protocol for CHMP2B antibody 12527-1-APDownload protocol
IF protocol for CHMP2B antibody 12527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier
pigWB,IF,IP

PLoS Pathog

Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.

Authors - Chun-Chun Liu
humanIF

Cell Death Dis

iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.

Authors - Kun Gao
humanWB

Oncotarget

Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.

Authors - Yi Huo
mouseIF

Sci Rep

VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.

Authors - Carolyn Ott
humanWB

Int J Parasitol Drugs Drug Resist

Characterization of Divalent Metal Transporter 1 (DMT1) in Brugia malayi suggests an intestinal-associated pathway for iron absorption.

Authors - Cristina Ballesteros