Anticorps Polyclonal de lapin anti-CDK2

• Phare
• Validé par KD/KO

CDK2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

269

Avis (1)

N° de cat : 10122-1-AP

Synonymes

p33 protein kinase, EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2

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Galerie de données de validation

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  WB analysis using 10122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10122-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10122-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10122-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 10122-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10122-1-AP

  IP result of anti-CDK2 (IP:10122-1-AP, 4ug; Detection:10122-1-AP 1:10000) with HeLa cells lysate 1200 ug.

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  IHC staining of human liver cancer using 10122-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10122-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10122-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10122-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10122-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 10122-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CDK2 antibody (10122-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10122-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CDK2 antibody (10122-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 10122-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CDK2 (10122-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A431, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3, tissu rénal de rat
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu testiculaire de souris,
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 262 publications below
• IHC: See 14 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 4 publications below

Informations sur le produit

10122-1-AP cible CDK2 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris, fish
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDK2 Protéine recombinante Ag0166
• Nom complet: cyclin-dependent kinase 2
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 30-33 kDa
• Numéro d’acquisition GenBank: BC003065
• Symbole du gène: CDK2
• Identification du gène (NCBI): 1017
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,CoIP	Signal Transduct Target Ther; SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis; Authors - Saisai Guo ; KD Validated
human	WB,IHC	Mol Cancer; LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.; Authors - Xi Jin ; KD Validated
human	WB	Sci Adv; Acquired resistance to KRAS G12C small-molecule inhibitors via genetic/nongenetic mechanisms in lung cancer; Authors - Atish Mohanty
human	WB	Nat Commun; Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.; Authors - Xiaoyu Ma
human	WB	J Clin Invest; TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.; Authors - Zheng Hu
human	WB	Nat Commun; Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets.; Authors - Jun Lu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Silvia (Verified Customer) (10-19-2021)

It works well for WB

• Applications: Western Blot
• Cell Tissue Type: Human fibroblasts

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