Anticorps Polyclonal de lapin anti-Calbindin-D28k

• Phare
• Validé par KD/KO

Calbindin-D28k Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, IP, ELISA

Conjugaison

Non conjugué

Publications

26

Avis (4)

N° de cat : 14479-1-AP

Synonymes

Calbindin, CALB1, D-28K, Calbindin D28, calbindin 1, 28kDa

---

Galerie de données de validation

• 

  WB analysis using 14479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 14479-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14479-1-AP (CALB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14479-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14479-1-AP (CALB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14479-1-AP

  IP result of anti-Calbindin-D28k (IP:14479-1-AP, 4ug; Detection:14479-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of hippocampus using 14479-1-AP

  IHC result of Calbindin antibody (14479-1-AP, 1:5000 in 1% BSA 0.3%Tx-100 Tris buffer incubated at 4℃ overnight) with freshly formalin fixed human hippocampus (floating section). Secondary antibody biotinylated anti-rabbit lgG 1:200 in 1% BSA 0.3%Tx-100 Tris buffer for 2 hours at room temperature. .

• 

  IHC staining of human cerebellum using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of human cerebellum using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of rat cerebellum using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14479-1-AP (Calbindin-D28k antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14479-1-AP (Calbindin-D28k antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse cerebellum using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), PAX6 antibody (67529-1-Ig, Clone: 2C5A1, red).

• 

  IF Staining of mouse cerebellum using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), PAX6 antibody (67529-1-Ig, Clone: 2C5A1, red).

• 

  IF Staining of mouse cerebellum using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), PTPRO antibody (67000-1-Ig, Clone: 2F2B4, red).

• 

  IF Staining of human kidney using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse kidney using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse kidney tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain, tissu rénal humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cervelet humain, hippocampe, tissu cérébral humain, tissu de cervelet de rat, tissu de cervelet de souris, tissu rénal de rat, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cervelet de souris, tissu rénal humain
• Résultats positifs en IF-Fro: tissu rénal de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 8 publications below
• IF: See 18 publications below

Informations sur le produit

14479-1-AP cible Calbindin-D28k dans les applications de WB, IHC, IF-P, IF-Fro, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Calbindin-D28k Protéine recombinante Ag5861
• Nom complet: calbindin 1, 28kDa
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC006478
• Symbole du gène: Calbindin
• Identification du gène (NCBI): 793
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

Publications

Species	Application	Title
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
mouse	IF	Cell; Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.; Authors - Haibo Zhou
mouse	IF	Nat Neurosci; Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage; Authors - Yao Wei
mouse	IF	Cell Death Differ; The long noncoding RNA Synage regulates synapse stability and neuronal function in the cerebellum.; Authors - Fei Wang
rat	IF	Autophagy; Autophagy and post-ischemic conditioning in retinal ischemia.; Authors - Biji Mathew
mouse	IHC,IF	Mol Ther; sEVsRVG selectively delivers antiviral siRNA to fetus brain, inhibits ZIKV infection and mitigates ZIKV-induced microcephaly in mouse model.; Authors - Rui Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Georgiana (Verified Customer) (09-11-2023)

Antibody labelled cell bodies and dendrites very clearly

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse brain

Calbindin-D28k Antibody Immunohistochemistry validation (1:1000 dilution) in mouse brain (Cat no:14479-1-AP)

FH

Ramzi (Verified Customer) (06-14-2023)

The brain vibratome sections were treated with Acid-Alcohol before the blocking solution.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: brain tissue ''Vibratome ''

Calbindin-D28k Antibody Immunofluorescence validation (1:300 dilution) in brain tissue ''Vibratome '' (Cat no:14479-1-AP)

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other

Produits associés

IMPA1 Polyclonal antibody

16593-1-AP

Calretinin Polyclonal antibody

12278-1-AP

RANBP9 Polyclonal antibody

51038-1-AP

TGM2 Polyclonal antibody

15100-1-AP

TRPV5 Polyclonal antibody

17322-1-AP

TRPV6 Polyclonal antibody

13411-1-AP