TGM2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TGM2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (1)

Cat no : 15100-1-AP

Synonyms

G ALPHA h, GNAH, TG(C), TG2, TGase 2, TGase C, TGase H, TGC, TGM2, Tissue transglutaminase, Transglutaminase 2, Transglutaminase C, Transglutaminase H

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Validation Data Gallery

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  WB analysis of HepG2 using 15100-1-AP

  WB result of TGM2 antibody (15100-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGM2 transfected HepG2 cells.

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  WB analysis using 15100-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15100-1-AP (TGM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 15100-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 15100-1-AP (TGM2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15100-1-AP

  IP result of anti-TGM2 (IP:15100-1-AP, 4ug; Detection:15100-1-AP 1:400) with HeLa cells lysate 1200ug.

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  IHC staining of human liver cancer using 15100-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15100-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15100-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15100-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HUVEC cells, HepG2 cells, BxPC-3 cells, HeLa cells, A549 cells, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 6 publications below
• IF: See 6 publications below
• ChIP: See 1 publications below

Product Information

15100-1-AP targets TGM2 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TGM2 fusion protein Ag7439
• Full Name: transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 77-80 kDa
• GenBank Accession Number: BC003551
• Gene Symbol: TGM2
• Gene ID (NCBI): 7052
• RRID: AB_2202885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		Nature; Autoimmune amelogenesis imperfecta in patients with APS-1 and coeliac disease; Authors - Yael Gruper
canine,human	IF	Nat Genet; New genes helped acorn barnacles adapt to a sessile lifestyle; Authors - Zhaofang Han
human	WB,IF	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
human	WB,IF	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB	Nat Commun; Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.; Authors - Chunqing Wang
human	WB,IHC	Cell Oncol (Dordr); Transglutaminases are oncogenic biomarkers in human cancers and therapeutic targeting of TGM2 blocks chemoresistance and macrophage infiltration in pancreatic cancer; Authors - Shan Zhang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jon (Verified Customer) (02-15-2022)

DILuted as suggested in %5 milk TBST blocking buffer. We have had problems with other TGM2 antibodies from other companies, but this one seemed no work better and yield a better signal. However, the apparent molecular weight of the TGM2 band appeared to be at a much lower MW than it is supposed to.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: EndoCßH1 human pancreatic beta cell line

TGM2 Antibody Western Blot validation (1:1000 dilution) in EndoCßH1 human pancreatic beta cell line (Cat no:15100-1-AP)