Calretinin Polyclonal antibody

Calretinin Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 12278-1-AP

Synonyms

29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR

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Validation Data Gallery

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  WB analysis using 12278-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis of human ileum using 12278-1-AP

  human ileum tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 12278-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12278-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 12278-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12278-1-AP

  IP result of anti-Calretinin (IP:12278-1-AP, 4ug; Detection:12278-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.

• 

  IHC staining of human appendicitis using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cerebellum using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cerebellum using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, human ileum tissue, human brain tissue, human colon tissue, mouse brain tissue, mouse colon tissue, rat brain tissue, rat colon tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human appendicitis tissue, mouse brain tissue, human brain tissue, human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 8 publications below

Product Information

12278-1-AP targets Calretinin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calretinin fusion protein Ag2924
• Full Name: calbindin 2
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC015484
• Gene Symbol: Calretinin
• Gene ID (NCBI): 794
• RRID: AB_2228338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

Publications

Species	Application	Title
human	IF	Cell Rep; Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment; Authors - Atsuko Yonemura
mouse	IF	Front Cell Dev Biol; Stepwise Induction of Inner Ear Hair Cells From Mouse Embryonic Fibroblasts via Mesenchymal- to-Epithelial Transition and Formation of Otic Epithelial Cells.; Authors - Qiong Yang
human	IF	Stem Cells Int; Characterization of Primary Cilia Formation in Human ESC-Derived Retinal Organoids; Authors - Ke Ning
human	IHC	Front Mol Biosci; Tumor Microenvironment Heterogeneity-Based Score System Predicts Clinical Prognosis and Response to Immune Checkpoint Blockade in Multiple Colorectal Cancer Cohorts.; Authors - Hufei Wang
mouse	IHC	Endocrinology; Fetal Bisphenol-A Induced Changes in Murine Behavior and Brain Gene Expression Persist in Aged Offspring.; Authors - Zhihao Wang
mouse	IF	Invest Ophthalmol Vis Sci; Primary Cilia in Amacrine Cells in Retinal Development.; Authors - Ke Ning