IMPA1 Polyclonal antibody

IMPA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16593-1-AP

Synonyms

IMPA, IMP1, IMP 1, EC:3.1.3.94, EC:3.1.3.25

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Validation Data Gallery

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  WB analysis of human kidney using 16593-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16593-1-AP (IMPA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 16593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16593-1-AP (IMPA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16593-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16593-1-AP (IMPA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 16593-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16593-1-AP (IMPA1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16593-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16593-1-AP (IMPA1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 16593-1-AP

  Immunofluorescent analysis of HepG2 cells, using IMPA1 antibody 16593-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: rat kidney tissue, human kidney tissue, human brain tissue, mouse kidney tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16593-1-AP targets IMPA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IMPA1 fusion protein Ag9632
• Full Name: inositol(myo)-1(or 4)-monophosphatase 1
• Calculated Molecular Weight: 277 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC008381
• Gene Symbol: IMPA1
• Gene ID (NCBI): 3612
• RRID: AB_2127216
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IMPA1 is an enzyme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1,4,5-trisphosphate and diacylglycerol.