Anticorps Polyclonal de lapin anti-AP3D1

AP3D1 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 16454-1-AP

Synonymes

ADTD, AP 3 complex subunit delta, AP 3 complex subunit delta 1, AP3D1, Delta adaptin, hBLVR

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Galerie de données de validation

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  WB analysis using 16454-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16454-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16454-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 16454-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 16454-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AP3D1 antibody (16454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules HepG2, cellules NIH/3T3
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

16454-1-AP cible AP3D1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AP3D1 Protéine recombinante Ag9390
• Nom complet: adaptor-related protein complex 3, delta 1 subunit
• Masse moléculaire calculée: 1153 aa, 130 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC010065
• Symbole du gène: AP3D1
• Identification du gène (NCBI): 8943
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3D1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

Publications

Species	Application	Title
mouse	WB,IF	Cancer Discov; Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation.; Authors - Wan Du
rat	IF	PLoS One; Adaptor protein-3: A key player in RBL-2H3 mast cell mediator release.; Authors - Elaine Zayas Marcelino da Silva

Produits associés

AP3B1 Polyclonal antibody

13384-1-AP

AP3B2 Polyclonal antibody

18331-1-AP

AP3M1 Polyclonal antibody

12114-1-AP

AP3M2 Polyclonal antibody

11925-1-AP

AP3S2 Polyclonal antibody

15319-1-AP