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AP3B2 Polyclonal antibody

AP3B2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 18331-1-AP

Synonyms

AP 3 complex subunit beta 2, AP3B2, Beta 3B adaptin, NAPTB

Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using AP3B2 Polyclonal antibody (18331-1-AP)

    WB analysis of rat brain using 18331-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 18331-1-AP (AP3B2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using AP3B2 Polyclonal antibody (18331-1-AP)

    WB analysis of mouse brain using 18331-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18331-1-AP (AP3B2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using AP3B2 Polyclonal antibody (18331-1-AP)

    IP experiment of mouse brain using 18331-1-AP

    IP result of anti-AP3B2 (IP:18331-1-AP, 4ug; Detection:18331-1-AP 1:500) with mouse brain tissue lysate 3600ug.

Tested Applications

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18331-1-AP is AP3B2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP3B2 fusion protein Ag12861
Full Name adaptor-related protein complex 3, beta 2 subunit
Calculated Molecular Weight 1082 aa, 119 kDa
Observed Molecular Weight 120-140 kDa
GenBank Accession NumberBC093739
Gene Symbol AP3B2
Gene ID (NCBI) 8120
RRIDAB_2878531
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3B2 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

Protocols

Product Specific Protocols
WB protocol for AP3B2 antibody 18331-1-APDownload protocol
IP protocol for AP3B2 antibody 18331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Neurology

Autoimmune gait disturbance accompanying adaptor protein-3B2-IgG.

Authors - Josephe A Honorat
View Article