AP3M2 Polyclonal antibody

• Featured Product
• KD/KO Validated

AP3M2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11925-1-AP

Synonyms

AP 3 complex subunit mu 2, AP3M2, AP47B, CLA20, HA1 47 kDa subunit homolog 2, Mu3B adaptin, P47B

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Validation Data Gallery

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  WB analysis of human brain using 11925-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11925-1-AP (AP3M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Hacat cells (siRNA) using 11925-1-AP

  WB result of AP3M2 antibody (11925-1-AP; 1:500) with siRNA cell lysate.

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  IP experiment of mouse brain using 11925-1-AP

  IP result of anti-AP3M2 (IP:11925-1-AP, 4ug; Detection:11925-1-AP 1:300) with mouse brain tissue lysate 3200 ug.

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  IHC staining of mouse brain using 11925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11925-1-AP (AP3M2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 11925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11925-1-AP (AP3M2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, Hacat cells (siRNA)
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11925-1-AP targets AP3M2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AP3M2 fusion protein Ag2572
• Full Name: adaptor-related protein complex 3, mu 2 subunit
• Calculated Molecular Weight: 418 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC056257
• Gene Symbol: AP3M2
• Gene ID (NCBI): 10947
• RRID: AB_10640438
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP3M2 is a 47-kDa protein that belongs to the adaptor complexes medium subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3M2 is a subunit of the AP-3 complex which is associated with the Golgi region as well as more peripheral structures. AP-3 is implicated in the transport of lysosomal membrane proteins.