AP3M1 Polyclonal antibody

• Featured Product
• KD/KO Validated

AP3M1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12114-1-AP

Synonyms

Mu-adaptin 3A, Mu3A-adaptin, AP-3 complex subunit mu-1, AP-3 adaptor complex mu3A subunit, Adaptor-related protein complex 3 subunit mu-1

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Validation Data Gallery

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  WB analysis using 12114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12114-1-AP (AP3M1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12114-1-AP (AP3M1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 12114-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12114-1-AP (AP3M1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 12114-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12114-1-AP (AP3M1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12114-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AP3M1 antibody (12114-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

12114-1-AP targets AP3M1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AP3M1 fusion protein Ag2759
• Full Name: adaptor-related protein complex 3, mu 1 subunit
• Calculated Molecular Weight: 418 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC026232
• Gene Symbol: AP3M1
• Gene ID (NCBI): 26985
• RRID: AB_2289629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3M1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

Publications

Species	Application	Title
human	WB	Elife; A novel GTP-binding protein-adaptor protein complex responsible for export of Vangl2 from the trans Golgi network.; Authors - Guo Yusong Y ; KD Validated
rat	WB	J Ethnopharmacol; Zhachong Shisanwei Pill resists ischemic stroke by lysosome pathway based on proteomics and bioinformatics; Authors - Qi Song

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