AP3S2 Polyclonal antibody

AP3S2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15319-1-AP

Synonyms

AP 3 complex subunit sigma 2, AP 3 complex subunit sigma 3B, AP3S2, AP3S3, Sigma 3B adaptin, Sigma adaptin 3b, sigma3b, Sigma3B adaptin

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Validation Data Gallery

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  WB analysis using 15319-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15319-1-AP (AP3S2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15319-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15319-1-AP (AP3S2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 15319-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15319-1-AP (AP3S2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15319-1-AP (AP3S2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary tumor using 15319-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15319-1-AP (AP3S2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, human testis tissue, mouse ovary tissue, mouse testis tissue, rat liver tissue
• Positive IHC detected in: human colon cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15319-1-AP targets AP3S2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AP3S2 fusion protein Ag7565
• Full Name: adaptor-related protein complex 3, sigma 2 subunit
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC002785
• Gene Symbol: AP3S2
• Gene ID (NCBI): 10239
• RRID: AB_2056644
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP3S2 is a 22-kDa protein that belongs to the adaptor complexes small subunit family. AP3S2 is a subunit of the AP-3 complex which is associated with the Golgi region as well as more peripheral structures. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-3 complex is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2).