Anticorps Polyclonal de lapin anti-AGER

• Phare
• Validé par KD/KO

AGER Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

34

N° de cat : 16346-1-AP

Synonymes

AGER, RAGE

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Galerie de données de validation

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  WB analysis using 16346-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16346-1-AP (AGER antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16346-1-AP (AGER antibody) at dilution of 1:4000 (under 10x lens).

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  IHC staining of mouse lung using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16346-1-AP (AGER antibody) at dilution of 1:4000 (under 40x lens).

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  IHC staining of human lung using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 16346-1-AP (AGER antibody) at dilution of 1:4000 (under 10x lens).

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  IHC staining of human lung using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 16346-1-AP (AGER antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IHC staining of human renal cell carcinoma using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 16346-1-AP (AGER Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 16346-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 16346-1-AP (AGER Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse lung using 16346-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using AGER antibody (16346-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, tissu pulmonaire de rat
• Résultats positifs en IHC: tissu pulmonaire de souris, tissu de carcinome à cellules rénales humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu pulmonaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IHC: See 8 publications below
• IF: See 8 publications below

Informations sur le produit

16346-1-AP cible AGER dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AGER Protéine recombinante Ag9540
• Nom complet: advanced glycosylation end product-specific receptor
• Masse moléculaire calculée: 404 aa, 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC020669
• Symbole du gène: AGER
• Identification du gène (NCBI): 177
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, inflammation, and certain diseases such as diabetes and Alzheimer's disease.

Publications

Species	Application	Title
mouse	WB	J Neuroinflammation; Celastrol exerts a neuroprotective effect by directly binding to HMGB1 protein in cerebral ischemia-reperfusion.; Authors - Dan-Dan Liu
rat	IF,WB	J Neuroinflammation; Inhibiting HMGB1-RAGE axis prevents pro-inflammatory macrophages/microglia polarization and affords neuroprotection after spinal cord injury.; Authors - Hong Fan
human,mouse	WB,IHC	Mol Med; AGEs promote atherosclerosis by increasing LDL transcytosis across endothelial cells via RAGE/NF-κB/Caveolin-1 pathway; Authors - Meng Shu ; KD Validated
mouse	WB	Front Pharmacol; Protective effect of heat-processed Gynostemma pentaphyllum on high fat diet-induced glucose metabolic disorders mice; Authors - Jin-Bo Xie
rat	WB,IF	FASEB J; PMID: 31961020; Authors - Shihong Wen
human	WB	Front Immunol; Roles of RAGE/ROCK1 Pathway in HMGB1-Induced Early Changes in Barrier Permeability of Human Pulmonary Microvascular Endothelial Cell.; Authors - Meng-Jiao Zhao ; KD Validated

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