APP Monoclonal antibody

APP Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a


human, pig, rat, rabbit and More (1)







Cat no : 60342-1-Ig


A4, AAA, ABETA, ABPP, AD1, AICD 50, AICD 57, AICD 59, AID(50), AID(57), AID(59), Amyloid beta A4 protein, amyloid P, amyloid precursor protein, Amyloid-beta precursor protein, APP, APPI, beta Amyloid, CTFgamma, CVAP, Gamma CTF(50) C31, PN II, PN2, PreA4, Protease nexin II, S APP alpha Soluble APP beta

Tested Applications

Positive WB detected inY79 cells, pig brain tissue, HEK-293 cells, rabbit brain tissue, rat brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60342-1-Ig targets APP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, pig, rat, rabbit samples.

Tested Reactivity human, pig, rat, rabbit
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen APP fusion protein Ag0769
Full Name amyloid beta (A4) precursor protein
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC004369
Gene Symbol APP
Gene ID (NCBI) 351
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1).


Product Specific Protocols
WB protocol for APP antibody 60342-1-IgDownload protocol
IHC protocol for APP antibody 60342-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Adv Sci (Weinh)

Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression

Authors - Rui Zhou

Aging Cell

Gamma frequency light flicker regulates amyloid precursor protein trafficking for reducing β-amyloid load in Alzheimer's disease model.

Authors - Qi Shen

Protein Cell

Metformin activates chaperone-mediated autophagy and improves disease pathologies in an Alzheimer disease mouse model.

Authors - Xiaoyan Xu

Cell Rep

CIP2A Causes Tau/APP Phosphorylation, Synaptopathy, and Memory Deficits in Alzheimer's Disease.

Authors - Yang-Ping Shentu

Aging (Albany NY)

Genomic deletion of TLR2 induces aggravated white matter damage and deteriorated neurobehavioral functions in mouse models of Alzheimer's disease.

Authors - Chao Zhou

Front Aging Neurosci

Agomelatine Prevents Amyloid Plaque Deposition, Tau Phosphorylation, and Neuroinflammation in APP/PS1 Mice.

Authors - Xiao-Bo Yang
  • KO Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Jinwei (Verified Customer) (10-01-2021)

The Beta Amyloid Monoclonal antibody works fine.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293
APP Antibody Western Blot validation (1:5000 dilution) in HEK293 (Cat no:60342-1-Ig)