APP Monoclonal antibody

• Featured Product
• KD/KO Validated

APP Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, rat, rabbit and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

21

CloneNo.

5C2A1

Reviews (1)

Cat no : 60342-1-Ig

Synonyms

A4, AAA, ABETA, ABPP, AD1, AICD 50, AICD 57, AICD 59, AID(50), AID(57), AID(59), Amyloid beta A4 protein, amyloid P, amyloid precursor protein, Amyloid-beta precursor protein, APP, APPI, beta Amyloid, CTFgamma, CVAP, Gamma CTF(50) C31, PN II, PN2, PreA4, Protease nexin II, S APP alpha Soluble APP beta

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Validation Data Gallery

• 

  WB analysis of Y79 using 60342-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 60342-1-Ig (APP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60342-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60342-1-Ig (APP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60342-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60342-1-Ig (APP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 60342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (APP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 60342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (APP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 60342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (Beta Amyloid antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 60342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (Beta Amyloid antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Y79 cells, pig brain tissue, HEK-293 cells, rabbit brain tissue, rat brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

60342-1-Ig targets APP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, pig, rat, rabbit samples.

• Tested Reactivity: human, pig, rat, rabbit
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: APP fusion protein Ag0769
• Full Name: amyloid beta (A4) precursor protein
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC004369
• Gene Symbol: APP
• Gene ID (NCBI): 351
• RRID: AB_2881451
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1).

Publications

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression; Authors - Rui Zhou
mouse	WB,IHC,IP	Aging Cell; Gamma frequency light flicker regulates amyloid precursor protein trafficking for reducing β-amyloid load in Alzheimer's disease model.; Authors - Qi Shen
human	WB	Protein Cell; Metformin activates chaperone-mediated autophagy and improves disease pathologies in an Alzheimer disease mouse model.; Authors - Xiaoyan Xu
human	WB	Cell Rep; CIP2A Causes Tau/APP Phosphorylation, Synaptopathy, and Memory Deficits in Alzheimer's Disease.; Authors - Yang-Ping Shentu
mouse	WB,IF	Aging (Albany NY); Genomic deletion of TLR2 induces aggravated white matter damage and deteriorated neurobehavioral functions in mouse models of Alzheimer's disease.; Authors - Chao Zhou
mouse	IF	Front Aging Neurosci; Agomelatine Prevents Amyloid Plaque Deposition, Tau Phosphorylation, and Neuroinflammation in APP/PS1 Mice.; Authors - Xiao-Bo Yang ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jinwei (Verified Customer) (10-01-2021)

The Beta Amyloid Monoclonal antibody works fine.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293

APP Antibody Western Blot validation (1:5000 dilution) in HEK293 (Cat no:60342-1-Ig)