HMGB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HMGB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

255

Reviews (2)

Cat no : 10829-1-AP

Synonyms

HMG-1, HMG1, HMG 1, High mobility group protein B1, High mobility group protein 1

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Validation Data Gallery

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  WB analysis using 10829-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10829-1-AP

  WB result of HMGB1 antibody (10829-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with negative control and HMGB1 knockout HeLa cells.

• 

  WB analysis of mouse kidney using 10829-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 10829-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 10829-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10829-1-AP (HMGB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 10829-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10829-1-AP (HMGB1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10829-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10829-1-AP (HMGB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10829-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10829-1-AP (HMGB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10829-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10829-1-AP (HMGB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 10829-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10829-1-AP (HMGB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse kidney tissue, RAW 264.7 cells, Jurkat cells, NIH/3T3 cells, C6 cells
• Positive IHC detected in: mouse brain tissue, human breast cancer tissue, human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 198 publications below
• IHC: See 50 publications below
• IF: See 62 publications below
• IP: See 8 publications below
• FC: See 1 publications below
• CoIP: See 4 publications below
• ChIP: See 2 publications below
• RIP: See 1 publications below

Product Information

10829-1-AP targets HMGB1 in WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HMGB1 fusion protein Ag1264
• Full Name: high-mobility group box 1
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC003378
• Gene Symbol: HMGB1
• Gene ID (NCBI): 3146
• RRID: AB_2232989
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling (23649808).This antibody is a rabbit polyclonal antibody raised against full length HMGB1 of human origin. The monomeric HMGB1 is 29 kDa and dimer is 58 kDa.

Publications

Species	Application	Title
mouse	IF	ACS Nano; Tumor-Acidity and Bioorthogonal Chemistry-Mediated On-Site Size Transformation Clustered Nanosystem to Overcome Hypoxic Resistance and Enhance Chemoimmunotherapy.; Authors - Kewei Wang
human,mouse	IHC,IF	ACS Nano; Mitochondria-Targeting Polymer Micelle of Dichloroacetate Induced Pyroptosis to Enhance Osteosarcoma Immunotherapy.; Authors - Jiakang Jin
mouse	WB	ACS Nano; Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy; Authors - Qiqi Zhang
mouse	IHC	Nat Commun; Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer.; Authors - Yanjuan Huang
mouse	IF	Small; An NIR-Fluorophore-Based Theranostic for Selective Initiation of Tumor Pyroptosis-Induced Immunotherapy.; Authors - Kewei Wang
human	WB	Nat Commun; VCP recruitment to mitochondria causes mitophagy impairment and neurodegeneration in models of Huntington's disease.; Authors - Xing Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chun (Verified Customer) (06-19-2022)

This antibody worked well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa cells

FH

PATRICK (Verified Customer) (12-01-2018)

This antibody worked well. ProteinTech provides antibody specific instructions that were helpful for each of the antibodies we ordered.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: PRIMARY ISOLATED ENDOTHELIAL CELLS

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