S100A12 Polyclonal antibody

S100A12 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16630-1-AP

Synonyms

CAAF1, CAGC, Calgranulin C, CGRP, EN RAGE, ENRAGE, MRP6, Neutrophil S100 protein, p6, Protein S100 A12, S100A12

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Validation Data Gallery

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  IHC staining of human tonsillitis using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human tonsillitis using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human tonsillitis using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 3 publications below

Product Information

16630-1-AP targets S100A12 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: S100A12 fusion protein Ag9961
• Full Name: S100 calcium binding protein A12
• Calculated Molecular Weight: 92 aa, 11 kDa
• GenBank Accession Number: BC070294
• Gene Symbol: S100A12
• Gene ID (NCBI): 6283
• RRID: AB_2878290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A12 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A12 is expressed abundantly in granulocytes, and has been implicated to play an important role on inflammatory reactions in various disease states.

Publications

Species	Application	Title
	IHC	Biol Reprod; Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signaling.; Authors - Claire Stenhouse
human	WB,IHC	Connect Tissue Res; S100A12 is involved in the pathology of osteoarthritis by promoting M1 macrophage polarization via the NF-κB pathway; Authors - Yi Zhang
human	IHC	Redox Biol; Lipoxin A4 ameliorates knee osteoarthritis progression in rats by antagonizing ferroptosis through activation of the ESR2/LPAR3/Nrf2 axis in synovial fibroblast-like synoviocytes; Authors - Zhehan Hu
	WB	Animals (Basel); Comparative Study on the Sperm Proteomes of Horses and Donkeys; Authors - Hong Ren