Anticorps Polyclonal de lapin anti-Aggrecan

Aggrecan Polyclonal Antibody for IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (4)

Applications

IHC, FC (Intra), ELISA, IF

Conjugaison

Non conjugué

Publications

247

N° de cat : 13880-1-AP

Synonymes

ACAN, Cartilage-specific proteoglycan core protein, Aggrecan core protein 2, Aggrecan 1, AGCAN

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Galerie de données de validation

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  IHC staining of human lung using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13880-1-AP (Aggrecan antibody at dilution of 1:400 (under 10x lens).

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  IHC staining of human lung using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13880-1-AP (Aggrecan antibody at dilution of 1:400 (under 40x lens).

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  IHC staining of human lung cancer using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13880-1-AP (Aggrecan antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13880-1-AP (Aggrecan antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cartilage using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human cartilage tissue slide using 13880-1-AP (Aggrecan antibody at dilution of 1:400 (under 10x lens).

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  IHC staining of human cartilage using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded human cartilage tissue slide using 13880-1-AP (Aggrecan antibody at dilution of 1:400 (under 40x lens).

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  IHC staining of mouse lung using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13880-1-AP (Aggrecan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 13880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13880-1-AP (Aggrecan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of ATDC-5 using 13880-1-AP

  1X10^6 ATDC-5 cells were intracellularly stained with 0.8 ug Anti-Human Aggrecan (13880-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IHC: tissu pulmonaire humain, tissu cartilagineux humain, tissu de cancer du poumon humain, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules ATDC-5,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 82 publications below
• IF: See 73 publications below

Informations sur le produit

13880-1-AP cible Aggrecan dans les applications de IHC, FC (Intra), ELISA, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, Lapin, poisson-zèbre, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Aggrecan Protéine recombinante Ag4985
• Nom complet: aggrecan
• Masse moléculaire calculée: 250 kDa
• Numéro d’acquisition GenBank: BC036445
• Symbole du gène: ACAN
• Identification du gène (NCBI): 176
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

Publications

Species	Application	Title
human,mouse	IHC,IF	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
human		Sci Transl Med; Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology; Authors - Su'an Tang
human		J Extracell Vesicles; miR-23a-3p-abundant small extracellular vesicles released from Gelma/nanoclay hydrogel for cartilage regeneration.; Authors - Hongxing Hu
rat	IF	Biomaterials; Injectable pathological microenvironment-responsive anti-inflammatory hydrogels for ameliorating intervertebral disc degeneration; Authors - Lei Liu
mouse,rat	IF	Cell Death Differ; Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation; Authors - Guangjin Chen
mouse		Nat Commun; Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease.; Authors - Mehdi Eshraghi

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