Elastin Polyclonal antibody

Elastin Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 15257-1-AP

Synonyms

ELN, WS, WBS, Tropoelastin, SVAS

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Validation Data Gallery

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  WB analysis using 15257-1-AP

  Human artery tissue were subjected to SDS PAGE followed by western blot with 15257-1-AP (Elastin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 15257-1-AP

  IP result of anti-Elastin (IP:15257-1-AP, 4ug; Detection:15257-1-AP 1:300) with human placenta tissue lysate 4000 ug.

• 

  IHC staining of human lung using 15257-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15257-1-AP (Elastin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15257-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15257-1-AP (Elastin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 15257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15257-1-AP (Elastin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 15257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15257-1-AP (Elastin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human artery tissue
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human lung tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Product Information

15257-1-AP targets Elastin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Elastin fusion protein Ag7179
• Full Name: elastin
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC065566
• Gene Symbol: Elastin
• Gene ID (NCBI): 2006
• RRID: AB_2878120
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Elastic fibers are an abundant and integral part of many extracellular matrices, in which they provide the elastic properties to tissues such as arterial, lung, and skin. Elastic fibers are consisting of an elastin core surrounded by a mantle of fibrillin-rich microfibrils (PMID: 12082143). Elastin is an extremely durable, insoluble biopolymer formed through the lysine-mediated crosslinking of its soluble precursor tropoelastin, which is an approximately 60-70 kDa protein (PMID: 15837523). Deletions and mutations in the elastin gene (ELN) are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa.

Publications

Species	Application	Title
human	WB	Am J Respir Cell Mol Biol; Glutaminolysis Promotes Collagen Translation and Stability via α-ketoglutarate Mediated mTOR Activation and Proline Hydroxylation.; Authors - Jing Ge
human	WB	J Med Chem; Design, Synthesis, and Biological Evaluation of an Orally Bioavailable, Potent, and Selective ROCK2 Inhibitor for Psoriasis Treatment; Authors - Yun Huang
human	WB	Int J Mol Sci; αvβ3 Integrin as a Link between the Development of Fibrosis and Thyroid Hormones in Systemic Sclerosis; Authors - Maia Yamila Kohon
		J Cell Physiol; Novel insight into the role of clusterin on intraocular pressure regulation by modifying actin polymerization and extracellular matrix remodeling in the trabecular meshwork.; Authors - Avinash Soundararajan
human	WB	Am J Physiol Cell Physiol; SEMA3B inhibits TGFβ-induced extracellular matrix protein production and its reduced levels are associated with a decline in lung function in IPF; Authors - Dan Jk Yombo
human	IF	Polymers (Basel); The Development of a 3D PET Fibrous Scaffold Modified with an Umbilical Cord dECM for Liver Tissue Engineering; Authors - Yang Li

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