Decorin Polyclonal antibody

• Featured Product
• KD/KO Validated

Decorin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zabrafish and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

26

Reviews (6)

Cat no : 14667-1-AP

Synonyms

DCN, CSCD, PGII, PG40, PG S2

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Validation Data Gallery

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  WB analysis using 14667-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14667-1-AP (Decorin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14667-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14667-1-AP (Decorin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14667-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14667-1-AP (Decorin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14667-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14667-1-AP (Decorin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 14667-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14667-1-AP (Decorin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14667-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14667-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14667-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 14667-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 14667-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 14667-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 14667-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 14667-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 14667-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 14667-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of zebrafish retina using 14667-1-AP

  Immunofluorescence analysis of Decorin polyclonal antibody (14667-1-AP, magenta) staining on tissue sections of the zebrafish retina at 4 days post fertilisation (dpf). Dilution 1:200. Tissue fixed overnight in 4% PFA. DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.

• 

  IF Staining of mouse liver using 14667-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Decorin antibody (14667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse liver using 14667-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Decorin antibody (14667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of BxPC-3 using 14667-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed BxPC-3 cells using Decorin antibody (14667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, MCF-7 cells, BxPC-3 cells, mouse colon tissue, rat heart tissue, NIH/3T3 cells
• Positive IHC detected in: human skin cancer tissue, human breast cancer tissue, human breast hyperplasia tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: zebrafish retina, mouse liver tissue
• Positive IF/ICC detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 4 publications below
• IF: See 5 publications below

Product Information

14667-1-AP targets Decorin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, zabrafish samples.

• Tested Reactivity: human, mouse, rat, zabrafish
• Cited Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Decorin fusion protein Ag6328
• Full Name: decorin
• Calculated Molecular Weight: 359 aa, 40 kDa
• Observed Molecular Weight: 70 kDa, 45-48 kDa
• GenBank Accession Number: BC005322
• Gene Symbol: Decorin
• Gene ID (NCBI): 1634
• RRID: AB_2090265
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.

Publications

Species	Application	Title
human	IF	Breast Cancer Res; Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors; Authors - Wei Yang
mouse	WB,IF	PLoS Genet; Impaired proteoglycan glycosylation, elevated TGF-β signaling, and abnormal osteoblast differentiation as the basis for bone fragility in a mouse model for gerodermia osteodysplastica.; Authors - Wing Lee Chan
mouse	IHC, WB	Cell Cycle; Negative impact of hyperglycaemia on mouse alveolar development.; Authors - Mei-Yao He
rat	WB	Stem Cell Res Ther; Extracellular vesicles produced by bone marrow mesenchymal stem cells attenuate renal fibrosis, in part by inhibiting the RhoA/ROCK pathway, in a UUO rat model.; Authors - Zhengzhou Shi
hamster	WB	Sci Rep; Cellular response to persistent foot-and-mouth disease virus infection is linked to specific types of alterations in the host cell transcriptome.; Authors - Lingling Han
human	IHC	J Cell Mol Med; Single-cell RNA sequencing analysis to characterize cells and gene expression landscapes in atrial septal defect.; Authors - Zunzhe Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (08-29-2023)

Antibody is working pretty well for IF on mouse tissue sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Liver

FH

Huai-Chin (Verified Customer) (10-24-2022)

Good antibody with only single band. Actual band size is higher than the calculated protein size which may indicated some protein modification.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: used on both mouse and human cell lines

FH

balawant (Verified Customer) (07-25-2022)

I have this antibody for my research it is working great in both tissue lysate as well as in cell lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: colon tissue and Caco2 cells

FH

Iram (Verified Customer) (06-13-2022)

Excellent antibody for western blotting

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: colon cancer cells

FH

Balawant (Verified Customer) (05-08-2022)

I have used this antibody. This antibody is excellent, and I used it for western blotting. The dilution of 1:1000 is functioning well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mice tissue and colon epithelial cells.

FH

Susmita (Verified Customer) (09-17-2020)

Antibody is working very good for WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 WB

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