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- KD/KO Validated
Biglycan Polyclonal antibody
Biglycan Polyclonal Antibody for IF, IHC, WB, ELISA
Cat no : 16409-1-AP
Validation Data Gallery
|Positive WB detected in||COLO 320 cells, mouse skeletal muscle tissue, HeLa cells, HepG2 cells, pig cartilage tissue|
|Positive IHC detected in||human lung tissue, human liver cancer tissue, human renal cell carcinoma tissue, human stomach cancer tissue, mouse lung tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 16409-1-AP is Biglycan Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Biglycan fusion protein Ag0683|
|Calculated molecular weight||42 kDa|
|Observed molecular weight||40-48 kDa|
|GenBank accession number||BC004244|
|Gene ID (NCBI)||633|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).
Landscape of cancer-associated fibroblasts identifies the secreted biglycan as a protumor and immunosuppressive factor in triple-negative breast cancer.
Biglycan Promotes Cancer Stem Cell Properties, NFκB Signaling and Metastatic Potential in Breast Cancer Cells.
Biglycan-mediated upregulation of MHC class I expression in HER-2/neu-transformed cells.
Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT-3 signalling pathway.
Establishment of a risk model by integrating hypoxia genes in predicting prognosis of esophageal squamous cell carcinoma
J Proteome Res
Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy