VCAM-1 Monoklonaler Antikörper

VCAM-1 Monoklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (1)

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

30

CloneNo.

1B12F12

Kat-Nr. : 66294-1-Ig

Synonyme

CD106, INCAM 100, V CAM 1, VCAM 1, VCAM1, VCAM-1

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Galerie der Validierungsdaten

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  IHC staining of human tonsillitis using 66294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66294-1-Ig (VCAM-1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66294-1-Ig (VCAM-1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66294-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66294-1-Ig (VCAM-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66294-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using VCAM-1 antibody (66294-1-Ig, Clone: 1B12F12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 66294-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using VCAM-1 antibody (66294-1-Ig, Clone: 1B12F12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

66294-1-Ig bindet in IHC, IF, ELISA VCAM-1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VCAM-1 fusion protein Ag24697
• Vollständiger Name: vascular cell adhesion molecule 1
• Berechnetes Molekulargewicht: 739 aa, 81 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC017276
• Gene symbol: VCAM1
• Gene ID (NCBI): 7412
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

Publikationen

Species	Application	Title
	IF	Redox Biol; Targeting mitochondria-inflammation circle by renal denervation reduces atheroprone endothelial phenotypes and atherosclerosis.; Authors - Zhuqing Li
human		J Hazard Mater; Amorphous silica nanoparticles induce inflammation via activation of NLRP3 inflammasome and HMGB1/TLR4/MYD88/NF-kb signaling pathway in HUVEC cells.; Authors - Xuting Liu
human		Int J Biol Macromol; Novel soybean polypeptide dglycin alleviates atherosclerosis in apolipoprotein E-deficient mice; Authors - Han Zhao
human		Life Sci; PMID: 29452166; Authors - Qiangbing Yang
human		Int J Mol Sci; Hypoxic Culture Maintains Cell Growth of the Primary Human Valve Interstitial Cells with Stemness.; Authors - Kaho Kanno
		J Nanobiotechnology; Macrophage-cancer hybrid membrane-coated nanoparticles for targeting lung metastasis in breast cancer therapy.; Authors - Chunai Gong

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