Alpha Tubulin Polyklonaler Antikörper

Alpha Tubulin Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (6)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

1403

Rezensionen (13)

Kat-Nr. : 11224-1-AP

Synonyme

TUBA1B, Tubulin alpha 1B chain, Tubulin alpha, EC:3.6.5.-, Detyrosinated tubulin alpha-1B chain

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• Galerie der Validierungsdaten
• Proteintech vs. Other Vendors

• 

  WB analysis using 11224-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11224-1-AP (alpha Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11224-1-AP

  IP result of anti-Alpha Tubulin (IP:11224-1-AP, 3ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 5000ug.

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  IHC staining of human colon cancer using 11224-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11224-1-AP (Alpha Tubulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11224-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11224-1-AP (Alpha Tubulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11224-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human endometrial cancer using 11224-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of Lymphatic endothelial cells using 11224-1-AP

  Lymphatic endothelial cells were stained for CD31 (green, Cat. No CL488-66065, 1:200) and alpha tubulin (magenta, Cat. 11224-1-AP, 1:200) with DAPI as a counterstain for visualizing the nucleus (yellow). CD31 is an endothelial cell adhesion protein and was conjugated to CoraLite-488 fluorescent dye to allow for direct visualization. Alpha tubulin is a cytoskeletal protein important in cell division and migration and was visualized with an Alexa Fluor 568 secondary antibody. Cells were fixed with 4% paraformaldehyde. Image credit: @Immunofluorescence on Instagram

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  IF Staining of HeLa using 11224-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (11224-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 11224-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11224-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 11224-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Endometriumkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen, Lymphendothelzellen Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1274 publications below
• IHC: See 4 publications below
• IF: See 86 publications below
• IP: See 11 publications below
• CoIP: See 1 publications below

Produktinformation

11224-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA Alpha Tubulin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, hamster, Huhn, Kaninchen, Maus, Ratte, Zebrafisch, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Alpha Tubulin fusion protein Ag1727
• Vollständiger Name: tubulin, alpha 1b
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC009314
• Gene symbol: Alpha Tubulin
• Gene ID (NCBI): 10376
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

What is the function of alpha tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.

 Where is alpha-tubulin expressed?

Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton. 

What are the post-translational modifications of alpha tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination¹, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.^2,3  Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

 1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).

2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).

3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).

Publikationen

Species	Application	Title
human	IF	Nat Biotechnol; Magnify is a universal molecular anchoring strategy for expansion microscopy; Authors - Aleksandra Klimas
human	WB	Cell Res; SARS-CoV-2 Z-RNA activates the ZBP1-RIPK3 pathway to promote virus-induced inflammatory responses; Authors - Shufen Li
human	WB	Nature; Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity.; Authors - Kuan-Chuan Pao
human	WB	Mol Cancer; A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis; Authors - Kaixuan Zeng
human	WB	Nat Med; Therapeutic efficacy of antisense oligonucleotides in mouse models of CLN3 Batten disease.; Authors - Jessica L Centa
	WB	Cell Host Microbe; Dietary fiber alleviates alcoholic liver injury via Bacteroides acidifaciens and subsequent ammonia detoxification; Authors - Haiyuan Shen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Samantha (Verified Customer) (05-10-2024)

Bands were faint but still visible when changes in contrast were applied. Some samples had big bands due to the large amounts present in the samples

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 5000
• Cell Tissue Type: Dorsal Root Ganglion, Spinal Cord

FH

Kamal (Verified Customer) (02-15-2024)

One of the best antibody to detect alpha-tubulin. Works well when diluted in 1X TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Mouse liver tissue

FH

Brogan (Verified Customer) (11-09-2022)

Used as a control for C elegans western blot

• Applications: Western Blot

FH

WEI (Verified Customer) (03-03-2022)

Good antibody for detect tubulin proteins

• Applications: Western Blot
• Primary Antibody Dilution: 1:8000
• Cell Tissue Type: Mouse Heart

FH

Maria (Verified Customer) (06-19-2021)

a Tubulin in human primary fibroblasts. 10 ug of total protein with DTT. 4-15 TGX gel. 3% BSA in PBST 1h RT. 1:1000 primary ab in BSA 3% in PBST (0.1% Tween) O/N 4ºC. Secondary ab 1:5000 1h RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human primary fibroblasts

Alpha Tubulin Antibody Western Blot validation (1:1000 dilution) in Human primary fibroblasts (Cat no:11224-1-AP)

FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed SH-SY5Y cells by Alpha Tubulin Polyclonal antibody at dilution of 1:100 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: SH-SY5Y cells

FH

Chao (Verified Customer) (03-12-2020)

Good sharp clear band in western blot.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

FH

Wei (Verified Customer) (01-30-2020)

Very good, sharp band for internal control.The protein is better than GAPDH for quantification in heart diseases.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse Heart

FH

KARTHIKEYAN (Verified Customer) (11-20-2019)

I used this antibody in mouse brain endothelial cell line, it worked really well, i would definitely recommend this product.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain Endothelial cell line

Alpha Tubulin Antibody Western Blot, validation (1:1000 dilution) in Brain Endothelial cell line (Cat no:11224-1-AP)

FH

Pooja (Verified Customer) (11-05-2019)

This antibody works very well as loading control. Even lower dilution can be use.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: MCF7 cells

FH

Yuan (Verified Customer) (11-02-2019)

Very nice staining for A549 cells with 1:200 dilution

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: A549 cell

Alpha Tubulin Antibody Immunofluorescence, validation (1:200 dilution) in A549 cell (Cat no:11224-1-AP)

FH

Kishor (Verified Customer) (01-30-2019)

I got good results for Western Blotting (1:1000) and IF (1:300)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1

Alpha Tubulin Antibody Western Blot,Immunofluorescence, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:11224-1-AP)

FH

kk (Verified Customer) (11-21-2018)

Excellent product, single and sharp band in all my blots

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: primary mouse islets

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