Beta Tubulin Polyclonal antibody

Beta Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

916

Reviews (6)

Cat no : 10094-1-AP

Synonyms

Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis using 10094-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10094-1-AP (Beta Tubulin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10094-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10094-1-AP (beta Tubulin antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10094-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10094-1-AP (beta Tubulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10094-1-AP

  IP result of anti-Beta Tubulin (IP:10094-1-AP, 3ug; Detection:10094-1-AP 1:1000) with mouse brain tissue lysate 7500ug.

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  IHC staining of human colon using 10094-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10094-1-AP (Beta Tubulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10094-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10094-1-AP (Beta Tubulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10094-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10094-1-AP (beta Tubulin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10094-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10094-1-AP (beta Tubulin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10094-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10094-1-AP (beta Tubulin antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 10094-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10094-1-AP (beta Tubulin antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of rat testis using 10094-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 10094-1-AP (beta Tubulin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of rat testis using 10094-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 10094-1-AP (beta Tubulin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cerebellum using 10094-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 10094-1-AP (beta Tubulin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cerebellum using 10094-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 10094-1-AP (beta Tubulin antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of mouse eye using 10094-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Beta Tubulin antibody (10094-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10094-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10094-1-AP (beta Tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10094-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (10094-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: U-251 cells, mouse kidney tissue, HEK-293 cells, rat kidney tissue, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon tissue, human cerebellum tissue, mouse brain tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse eye tissue
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 865 publications below
• IHC: See 1 publications below
• IF: See 30 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below

Product Information

10094-1-AP targets Beta Tubulin in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit, chicken, zebrafish, hamster, sheep, goat, fish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Beta Tubulin fusion protein Ag0136
• Full Name: tubulin, beta 3
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC000748
• Gene Symbol: TUBB3
• Gene ID (NCBI): 10381
• RRID: AB_2210695
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

Publications

Species	Application	Title
human	IF	Nat Biotechnol; Magnify is a universal molecular anchoring strategy for expansion microscopy; Authors - Aleksandra Klimas
mouse	WB	Nat Nanotechnol; Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades.; Authors - Xuefei Lu
mouse	WB	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
mouse	WB	Cell Metab; Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue.; Authors - Marco Rosina
human	IF	Nat Cell Biol; Single-cell multi-omics profiling of human preimplantation embryos identifies cytoskeletal defects during embryonic arrest; Authors - Teng Wang
human	WB	Nat Immunol; The tumor suppressor PTEN has a critical role in antiviral innate immunity.; Authors - Shun Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

PK (Verified Customer) (08-14-2024)

excellent

• Applications: Western Blot
• Primary Antibody Dilution: 3000
• Cell Tissue Type: AC16

Beta Tubulin Antibody Western Blot validation (3000 dilution) in AC16 (Cat no:10094-1-AP)

FH

Susanne (Verified Customer) (11-25-2022)

• Applications: Western Blot
• Primary Antibody Dilution: 1 : 1000
• Cell Tissue Type: human dermal fibroblasts (lysates)

Beta Tubulin Antibody Western Blot validation (1 : 1000 dilution) in human dermal fibroblasts (lysates) (Cat no:10094-1-AP)

FH

Macarena (Verified Customer) (10-07-2022)

excellent results. No non specific binding. The loading control of choice in my lab

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: cell lines

FH

parisa (Verified Customer) (12-03-2021)

We had the anti-mouse antibody and tried this anti-rabbit antibody which worked very well and will be helpful for us.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293T

FH

Azita (Verified Customer) (06-02-2021)

Immunohistochemistry labelling of (4% PFA) fixed mice spinal cord tissues using Beta Tubulin Polyclonal Antibody at dilution of 1:50 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: mice spinal cord tissues

FH

Elise (Verified Customer) (12-27-2020)

Works perfectly well!

• Applications: Western Blot
• Cell Tissue Type: Human spermatozoa

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