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TRAF6 Monoklonaler Antikörper
TRAF6 Monoklonal Antikörper für FC, IF, WB, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human und mehr (2)
Anwendung
WB, IP, IHC, IF, FC, ELISA
Konjugation
Unkonjugiert
CloneNo.
1D1E1
Kat-Nr. : 66498-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, U2OS-Zellen |
Erfolgreiche Detektion in IF | HeLa-Zellen |
Erfolgreiche Detektion in FC | Jurkat-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunfluoreszenz (IF) | IF : 1:200-1:800 |
Durchflusszytometrie (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 4 publications below |
WB | See 50 publications below |
IHC | See 3 publications below |
IF | See 5 publications below |
IP | See 3 publications below |
Produktinformation
66498-1-Ig bindet in WB, IP, IHC, IF, FC, ELISA TRAF6 und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human, Fisch, Hausschwein |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | TRAF6 fusion protein Ag24696 |
Vollständiger Name | TNF receptor-associated factor 6 |
Berechnetes Molekulargewicht | 522 aa, 60 kDa |
Beobachtetes Molekulargewicht | 60 kDa |
GenBank-Zugangsnummer | BC031052 |
Gene symbol | TRAF6 |
Gene ID (NCBI) | 7189 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for TRAF6 antibody 66498-1-Ig | Protokoll herunterladen |
IHC protocol for TRAF6 antibody 66498-1-Ig | Protokoll herunterladen |
IF protocol for TRAF6 antibody 66498-1-Ig | Protokoll herunterladen |
FC protocol for TRAF6 antibody 66498-1-Ig | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Autophagy Increased alveolar epithelial TRAF6 via autophagy-dependent TRIM37 degradation mediates particulate matter-induced lung metastasis | ||
Cell Death Differ Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling | ||
J Immunother Cancer Helicobacter pylori infection disturbs the tumor immune microenvironment and is associated with a discrepant prognosis in gastric de novo diffuse large B-cell lymphoma. | ||
Br J Pharmacol Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A | ||
Theranostics Fibrinogen-like protein 2 aggravates nonalcoholic steatohepatitis via interaction with TLR4, eliciting inflammation in macrophages and inducing hepatic lipid metabolism disorder. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tanusree (Verified Customer) (12-03-2019) | This antibody works good in western blotting analysis using mouse tissues.
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FH Andrea (Verified Customer) (12-07-2018) | Antibody works well for western blot at a dilution of 1:8000. Did not work for immunoprecipitation.
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