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TRAF6 Monoklonaler Antikörper

TRAF6 Monoklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D1E1

Kat-Nr. : 66498-1-Ig

Synonyme

RING finger protein 85, RNF85, TRAF6



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, U2OS-Zellen
Erfolgreiche Detektion in IFHeLa-Zellen
Erfolgreiche Detektion in FCJurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:20000
Immunfluoreszenz (IF)IF : 1:200-1:800
Durchflusszytometrie (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66498-1-Ig bindet in WB, IP, IHC, IF, FC, ELISA TRAF6 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Fisch, Hausschwein
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen TRAF6 fusion protein Ag24696
Vollständiger Name TNF receptor-associated factor 6
Berechnetes Molekulargewicht 522 aa, 60 kDa
Beobachtetes Molekulargewicht 60 kDa
GenBank-ZugangsnummerBC031052
Gene symbol TRAF6
Gene ID (NCBI) 7189
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.

Protokolle

Produktspezifische Protokolle
WB protocol for TRAF6 antibody 66498-1-IgProtokoll herunterladen
IHC protocol for TRAF6 antibody 66498-1-IgProtokoll herunterladen
IF protocol for TRAF6 antibody 66498-1-IgProtokoll herunterladen
FC protocol for TRAF6 antibody 66498-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IF

Autophagy

Increased alveolar epithelial TRAF6 via autophagy-dependent TRIM37 degradation mediates particulate matter-induced lung metastasis

Authors - Jiajun Liu
humanWB

EBioMedicine

PMID: 31594754

Authors - Yiyi Liu
humanWB

Cell Death Differ

Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling

Authors - Shunfeng Hu
humanIHC

J Immunother Cancer

Helicobacter pylori infection disturbs the tumor immune microenvironment and is associated with a discrepant prognosis in gastric de novo diffuse large B-cell lymphoma.

Authors - Yuwei Deng
humanWB

Br J Pharmacol

Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A

Authors - Lu Zhang
WB

Theranostics

Fibrinogen-like protein 2 aggravates nonalcoholic steatohepatitis via interaction with TLR4, eliciting inflammation in macrophages and inducing hepatic lipid metabolism disorder.

Authors - Junjian Hu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain
FH

Andrea (Verified Customer) (12-07-2018)

Antibody works well for western blot at a dilution of 1:8000. Did not work for immunoprecipitation.

  • Applications: Western Blot, Immunoprecipitation,