TANK Polyclonal antibody

TANK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 27065-1-AP

Synonyms

I TRAF, ITRAF, TANK, TRAF interacting protein, TRAF2

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Validation Data Gallery

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  WB analysis of THP-1 using 27065-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 27065-1-AP (TANK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27065-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27065-1-AP (TANK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27065-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27065-1-AP (TANK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: THP-1 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

The immunogen of 27065-1-AP is TANK Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TANK fusion protein Ag25740
• Full Name: TRAF family member-associated NFKB activator
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC003388
• Gene Symbol: TANK
• Gene ID (NCBI): 10010
• RRID: AB_2880738
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cancer Res; FIP200 suppresses immune checkpoint therapy responses in breast cancers by limiting AZI2/TBK1/IRF signaling independent of its canonical autophagy function.; Authors - Takako Okamoto
rat	WB	Int Immunopharmacol; The effect of Astragaloside IV on ethanol-induced gastric mucosal injury in rats: Involvement of inflammation.; Authors - Shumin Qin
human	WB	J Mol Cell Biol; TRIM14 promotes endothelial activation via activating NF-κB signaling pathway.; Authors - Xuan Huang