TAK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TAK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, hamster and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

45

Cat no : 12330-2-AP

Synonyms

MAP3K7, TGF beta activated kinase 1, Mitogen-activated protein kinase kinase kinase 7, mitogen activated protein kinase kinase kinase 7, MAP3K 7

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 12330-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12330-2-AP

  WB result of TAK1 antibody (12330-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAK1 transfected NIH/3T3 cells.

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  WB analysis of A431 using 12330-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 12330-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12330-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12330-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12330-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12330-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 12330-2-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12330-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of CHO using 12330-2-AP

  CHO cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12330-2-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12330-2-AP (TAK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 12330-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12330-2-AP (TAK1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12330-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12330-2-AP (TAK1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12330-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12330-2-AP (TAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12330-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12330-2-AP (TAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12330-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12330-2-AP (TAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12330-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12330-2-AP (TAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of NIH/3T3 using 12330-2-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using TAK1 antibody (12330-2-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, K-562 cells, SKOV-3 cells, COLO 320 cells, A375 cells, A431 cells, CHO cells, MCF-7 cells, HeLa cells, mouse heart tissue, A2780 cells
• Positive IHC detected in: human prostate cancer tissue, human brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 42 publications below
• IF: See 1 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below

Product Information

12330-2-AP targets TAK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, hamster samples.

• Tested Reactivity: human, mouse, hamster
• Cited Reactivity: human, mouse, rat, pig, fish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TAK1 fusion protein Ag3041
• Full Name: mitogen-activated protein kinase kinase kinase 7
• Calculated Molecular Weight: 579 aa, 64 kDa
• Observed Molecular Weight: 64-67 kDa, 75-80 kDa
• GenBank Accession Number: BC017715
• Gene Symbol: TAK1
• Gene ID (NCBI): 6885
• RRID: AB_2140101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transforming growth factor-β (TGF-β)-activated kinase 1 (TAK1), also known as MAP3K7-mitogen-activated protein kinase kinase kinase 7-  is a serine/threonine  protein kinase triggering phosphorylation of AMP-activated protein kinase (AMPK) and playing a role in the signal transduction of TGF-β, bone morphogenetic  proteins (BMPs), and various proinflammatory mediators.

What is the molecular weight of TAK1? Are there any isoforms of TAK1?

The molecular weight of full-length TAK1 is 55 kDa. There are four splicing variants of TAK1. TAK1-B represents the full-length variant, while TAK1-A, TAK1-C, and TAK1-D lack exons 12 and/or 16. TAK-1 variants differ in their expression levels across tissues (PMID: 11118615).

Is TAK1 post-translationally modified?

TAK1 kinase activity is modulated by interacting with TAK1-binding proteins (TABs). Binding  to  TABs  triggers autophosphorylation of TAK1 and depends on  polyubiquitination of receptor-interacting protein 1 (RIP1) (PMID: 8638164 and 11460167). Additionally, TAK1 can be ubiquitinated, acetylated, and O-glycosylated, which impacts its activity (PMID: 28106845).

What is the subcellular localization of TAK1?

TAK1 is present in the cytoplasm.

What is the tissue expression pattern of TAK1?

TAK1 is ubiquitously expressed.

How can TAK1 activity be monitored?

TAK1 requires phosphorylation for its activity. TAK1 autophosphorylation in the presence of TBPs occurs on Thr178, Thr184, Thr187, and Ser192 residues. The most commonly used phosphorylation readout is Thr187 phosphorylation.

Publications

Species	Application	Title
human	WB	EMBO J; CARS senses cysteine deprivation to activate AMPK for cell survival.; Authors - Mengqiu Yuan ; KD Validated
mouse	WB	Cell Death Differ; Protein phosphatase 6 is a key factor regulating spermatogenesis.; Authors - Wen-Long Lei
mouse	WB	Diabetes; Hepatic P38 Activation Modulates Systemic Metabolism Through Fgf21-Mediated Interorgan Communication.; Authors - Wei Liu
human	WB	EMBO Rep; Mycobacterium tuberculosis protein kinase G acts as an unusual ubiquitinating enzyme to impair host immunity.; Authors - Jing Wang
mouse	WB	PLoS Pathog; Inducible LGALS3BP/90K activates antiviral innate immune responses by targeting TRAF6 and TRAF3 complex.; Authors - Gang Xu
mouse	WB	Food Funct; DHA-phospholipids (DHA-PL) and EPA-phospholipids (EPA-PL) prevent intestinal dysfunction induced by chronic stress; Authors - Wanxiu Cao