MYD88 Polyclonal antibody

• Featured Product
• KD/KO Validated

MYD88 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

140

Reviews (2)

Cat no : 23230-1-AP

Synonyms

MYD88, MYD88D

---

Validation Data Gallery

• 

  WB analysis using 23230-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23230-1-AP (MYD88 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23230-1-AP

  WB result of MYD88 antibody (23230-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYD88 transfected HepG2 cells.

• 

  WB analysis of MCF-7 using 23230-1-AP

  WB result of MYD88 antibody (23230-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYD88 transfected MCF-7 cells.

---

Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, MCF-7 cells, Jurkat cells, K-562 cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 131 publications below
• IHC: See 8 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

23230-1-AP targets MYD88 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, sheep, monkey, pig, canine, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYD88 fusion protein Ag19770
• Full Name: myeloid differentiation primary response gene (88)
• Calculated molecular weight: 33 kDa
• Observed molecular weight: 33 kDa
• GenBank accession number: BC013589
• Gene symbol: MYD88
• Gene ID (NCBI): 4615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myeloid differentiation primary response 88 (MYD88) is an adapter protein critical to the innate and adaptive immune response.

 What is the molecular weight of MYD88?

The molecular weight of MYD88 is 33 kDa.

 What is the cellular localization of MYD88?

The subcellular localization of MYD88 is largely confined to the cytoplasm as condensed forms or aggregated structures.

 What is the role of MYD88 in the IL-1R signaling pathway?

MYD88 plays a major role in the inflammatory signaling pathways downstream of Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) families. MYD88 links these receptors to IL-1R-associated kinases (IRAK), such as IRAK1 and IRAK2, via protein-protein interactions. The C-terminal TIR domain of MYD88 mediates the interaction with the receptors, whereas the N-terminal death domain of MYD88 associates with IRAK family members (PMID: 25580251). MYD88 acts via its intermediate domain to phosphorylate and thus activate IRAK1, IRAK2, IRF7, and TRAF6 to trigger NF-kappa-B signaling and cytokine secretion as part of the inflammatory response (PMID: 19679662).

 What is MYD88's involvement in disease?

Defects in MYD88 due to deficiency of the protein leads to recurrent pyogenic bacterial infections, including invasive pneumococcal disease. Patients usually die between 1 and 11 months of age, but surviving patients are otherwise healthy with normal resistance to other microbes (PMID: 18669862). Mutations in the MYD88 gene also lead to the development of cancers such as lymphoma (PMID: 21179087) and some autoimmune disorders like ulcerative colitis (PMID: 24189845).

Publications

Species	Application	Title
human	WB	Gut Microbes; Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway; Authors - Jian-Wei Zhang
pig	WB	Nucleic Acids Res; The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.; Authors - Xin Zong
mouse	WB	J Crohns Colitis; A Molecular Targeted Immunotherapeutic Strategy for Ulcerative Colitis via Dual-targeting Nanoparticles Delivering miR-146b to Intestinal Macrophages.; Authors - Feihong Deng
mouse	WB	Theranostics; Prussian blue nanozyme-mediated nanoscavenger ameliorates acute pancreatitis via inhibiting TLRs/NF-κB signaling pathway.; Authors - Xue Xie
mouse	IHC	Theranostics; Depletion of the diabetic gut microbiota resistance enhances stem cells therapy in type 1 diabetes mellitus.; Authors - Wanqi Lv
mouse	WB	Oxid Med Cell Longev; Aloin Preconditioning Attenuates Hepatic Ischemia/Reperfusion Injury via Inhibiting TLR4/MyD88/NF-κB Signal Pathway In Vivo and In Vitro.; Authors - Yichao Du

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Rouba (Verified Customer) (07-05-2022)

Antibody was diluted to the 1/100 in blocking solution and incubated ON at 4 degrees celcius

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Myoblasts

MYD88 Antibody Immunofluorescence validation (1/100 dilution) in Myoblasts (Cat no:23230-1-AP)

FH

Cassand (Verified Customer) (10-22-2021)

Nice antibody works good.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Gut

MYD88 Antibody Immunohistochemistry validation (1:300 dilution) in Gut (Cat no:23230-1-AP)