TRAF3 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRAF3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IF, IP, ELISA

Conjugate

Unconjugated

Publications

25

Cat no : 18099-1-AP

Synonyms

CAP 1, CAP1, CD40 binding protein, CD40bp, CRAF1, LAP1, LMP1 associated protein 1, TRAF3

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Validation Data Gallery

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  WB analysis using 18099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18099-1-AP (TRAF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18099-1-AP

  WB result of TRAF3 antibody (18099-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAF3 transfected HEK-293 cells.

• 

  WB analysis using 18099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18099-1-AP (TRAF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of multi-cells using 18099-1-AP

  WB results of 18099-1-aP (anti TRAF3).

• 

  WB analysis of HEK-293 using 18099-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18099-1-AP (TRAF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 18099-1-AP

  IP result of anti-TRAF3 (IP:18099-1-AP, 4ug; Detection:18099-1-AP 1:1000) with Raji cells lysate 3160 ug.

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Tested Applications

• Positive WB detected in: COLO 320 cells, HEK-293 cells, multi-cells, HeLa cells, Raji cells, NIH/3T3 cells
• Positive IP detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 23 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

18099-1-AP targets TRAF3 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAF3 fusion protein Ag12185
• Full Name: TNF receptor-associated factor 3
• Calculated Molecular Weight: 568 aa, 64 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC075087
• Gene Symbol: TRAF3
• Gene ID (NCBI): 7187
• RRID: AB_10837364
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNF receptor-associated factor 3 (TRAF3) has many alternative names including CAP-1, CD40 receptor-associated factor 1(CRAF1), CD40-binding protein(CD40BP) and LMP1-associated protein 1 (LAP1). TRAF3 regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of innate immune response. It modulates signaling pathways leading to the production of cytokines and interferon. As an essential constituent of several E3 ubiquitin-protein ligase complexes, TRAF3 promote 'Lys-63'-linked ubiquitination of target proteins. Moreover, TRAF3 undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to various stimuli and is deubiquitinated by OTUB1, OTUB2 and OTUD5. Several alternatively spliced transcript variants encoding distinct isoforms (64 kDa and 55 kDa) have been reported.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng ; KD Validated
		Cell Death Differ; The E3 ligase ASB3 downregulates antiviral innate immunity by targeting MAVS for ubiquitin-proteasomal degradation; Authors - Mingyang Cheng
mouse	WB	J Exp Med; TRIM24 facilitates antiviral immunity through mediating K63-linked TRAF3 ubiquitination.; Authors - Qingchen Zhu
pig	WB	Nucleic Acids Res; The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.; Authors - Xin Zong
human	WB	EMBO J; Dimer-specific immunoprecipitation of active caspase-2 identifies TRAF proteins as novel activators.; Authors - Alexander C Robeson ; KD Validated
mouse	WB,IP	EMBO Rep; Estrogen receptor alpha signaling in dendritic cells modulates autoimmune disease phenotype in mice; Authors - Yee Ming Khaw

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