TOM20 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TOM20 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Huhn, human, Maus, Ratte und mehr (5)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

529

Rezensionen (21)

Kat-Nr. : 11802-1-AP

Synonyme

TOMM20, TOMM 20, tom 20, MOM19, MAS20

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Galerie der Validierungsdaten

• 

  WB analysis using 11802-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11802-1-AP (TOM20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11802-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11802-1-AP (TOM20 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11802-1-AP

  IP result of anti-TOM20 (IP:11802-1-AP, 4ug; Detection:11802-1-AP 1:6000) with HeLa cells lysate 1085 ug.

• 

  IHC staining of mouse liver using 11802-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11802-1-AP (TOM20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 11802-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11802-1-AP (TOM20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11802-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11802-1-AP (TOM20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11802-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11802-1-AP (TOM20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 11802-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using TOM20 antibody (11802-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 11802-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11802-1-AP (TOM20 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Mausnierengewebe, Rattennierengewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HUVEC-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 326 publications below
• IHC: See 18 publications below
• IF: See 256 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

11802-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA TOM20 und zeigt Reaktivität mit Huhn, human, Maus, Ratten

• Getestete Reaktivität: Huhn, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, hamster, Hausschwein, Huhn, Kaninchen, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TOM20 fusion protein Ag2378
• Vollständiger Name: translocase of outer mitochondrial membrane 20 homolog (yeast)
• Berechnetes Molekulargewicht: 145 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC000882
• Gene symbol: TOM20
• Gene ID (NCBI): 9804
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Background

 TOM20 (KIAA0016) belongs to the Tom family. It is a subunit of the mitochondrial import receptor (PMID: 7584026), whose main role is to translocate cytosolically synthesized mitochondrial proteins through the outer mitochondrial membrane and subsequently facilitate the movement of proteins into the TOM40 translocation pore complex (PMID: 21173275).

 What is the molecular weight of TOM20? 

 It is a short 16.3 kDa protein containing several highly conserved regions, including the transmembrane segment, the ligand-binding domain, and flexible segments at the N terminus and the C terminus of the protein crucial for its function (PMID: 15733919).

 What is the subcellular localization of TOM20?

 It specifically localizes in the mitochondrial outer membrane. In malignant cells, strong granular staining in the cytoplasm has been observed.

 What is the tissue specificity of TOM20?

 It is ubiquitously expressed in various tissues, with a particularly high expression in the brain, thyroid, and pancreas.

 What is the function of TOM20 in the mitochondrial membrane?

 Mitochondrial preproteins are generally synthesized in the cytoplasm with signal or targeting sequences, which are recognized by specific receptors on the outer mitochondrial membrane. TOM20, as one of the components of the TOM40 complex, specifically recognizes pre-sequences on target proteins or their unfolded forms. In addition to its translocase activity, TOM20 may act as a chaperone preventing these proteins from aggregation at the surface of mitochondria (PMID: 14699115).

 What is TOM20's involvement in disease?

 Diseases linked to the misregulation of TOM20 include Perry Syndrome and neurodegeneration with brain iron accumulation 2A. Numerous studies also associate deregulation of TOM20 with an array of mitochondrial dysfunctions and mitophagy defects (PMIDs: 30254015, 30160596). 

Publikationen

Species	Application	Title
	IF	Nature; Stress response silencing by an E3 ligase mutated in neurodegeneration; Authors - Diane L Haakonsen
mouse	WB	Nature; CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization; Authors - Kevin Qian
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
mouse	WB	Nature; BCAA catabolism in brown fat controls energy homeostasis through SLC25A44.; Authors - Takeshi Yoneshiro
mouse	IF	Nature; Low-dose metformin targets the lysosomal AMPK pathway through PEN2.; Authors - Teng Ma
human	WB	Science; Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms.; Authors - David E Gordon

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

PK (Verified Customer) (07-05-2024)

Excellent

• Applications: Western Blot
• Primary Antibody Dilution: 2000
• Cell Tissue Type: l2

TOM20 Antibody Western Blot validation (2000 dilution) in l2 (Cat no:11802-1-AP)

FH

SD (Verified Customer) (07-05-2024)

Excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: HEK

TOM20 Antibody Western Blot validation (1000 dilution) in HEK (Cat no:11802-1-AP)

FH

Joel (Verified Customer) (11-07-2023)

Works well for immunofluorescence (1:200) and Western blotting (1:1000-1:2000)

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human and mouse cancer cell lines

FH

Lisa (Verified Customer) (03-23-2023)

The antibody worked very well for immunofluorescence, combined with a secondary fluorescent antibody 555.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: COS-7

TOM20 Antibody Immunofluorescence validation (1:200 dilution) in COS-7 (Cat no:11802-1-AP)

FH

S (Verified Customer) (12-12-2022)

excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: FIBROBLAST

TOM20 Antibody Western Blot validation (1000 dilution) in FIBROBLAST (Cat no:11802-1-AP)

FH

Kazu (Verified Customer) (11-22-2022)

This antibody works and provides robust signals of TOM20 immunoreactivity on feline tissue sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Optic nerve

FH

Wan (Verified Customer) (03-01-2022)

We got very clear and correct bands for tom20., very good antibody.

• Applications: Western Blot

FH

Süleyman (Verified Customer) (02-06-2022)

It is very specific and nicely labelled mitochondria. I highly suggest it. 4% paraformaldehyde fixation for 10 min at RT and 0.3% Triton-x100 10 minutes for permeabilization at RT. I used 2h at RT or 16h at 4 degree incubation. Alexa fluor 488 1:700 is used for 1 hour at RT for secondary ab.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa

TOM20 Antibody Immunofluorescence validation (1:100 dilution) in HeLa (Cat no:11802-1-AP)

FH

Sahil (Verified Customer) (10-09-2020)

The tom 20 works great. We wanted to compare and it does stand out

• Applications: Immunohistochemistry

FH

Thomas (Verified Customer) (09-18-2020)

The antibody allows for exceptionally clear visualisation of the mitochondrial outer membrane. HeLa cells were fixed in 4% PFA for 15 mins and permeabilised in 0.1% Triton-X 100 in PBS. Cells were blocked in 1% BSA. Primary antibody solution was diluted at 1:250 in blocking solution and incubated for 1 hour. Goat anti-rabbit 488 Alexa Fluor secondary antibody (1:250 - green) and DAPI (1:2000 - blue) were incubated with cells for 1 hour.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HeLa

TOM20 Antibody Immunofluorescence validation (1:250 dilution) in HeLa (Cat no:11802-1-AP)

FH

Maria (Verified Customer) (06-05-2020)

TOM20 1:200 in PBST (0.1% triton) + 10% Donkey Serum O/N 4ºC incubation. Donkey anti-rabbit alexa fluor 555 1:500 1h RT incubation.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human fibroblasts

TOM20 Antibody Immunofluorescence validation (1:200 dilution) in Human fibroblasts (Cat no:11802-1-AP)

FH

Mohammad (Verified Customer) (02-25-2020)

For mouse hippocampusWorks well

• Applications: Immunofluorescence,

FH

Uxoa (Verified Customer) (02-18-2020)

TOM20 ab is great for ICC and WB. Tested in human primary fibroblats. ICC: fixed fibroblats with 4% PFA 15min. TOM20 diluted 1:200 in PBST (0.1% triton) + 10% DS O/N 4ºC incubation. Donkey anti-rabbit alexa fluor 488 1:500 1h RT. DAPI in blue.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human fibroblasts

TOM20 Antibody Western Blot,Immunofluorescence, validation (1:200 dilution) in Human fibroblasts (Cat no:11802-1-AP)

FH

Benjamin (Verified Customer) (01-10-2020)

Reliably used in western blot where it identifies the correct band with high specificity.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293T

FH

Aamir (Verified Customer) (01-08-2020)

WB worked fine

• Applications: Western Blot,
• Primary Antibody Dilution: 1000
• Cell Tissue Type: HEKs

FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

Fulong (Verified Customer) (10-24-2019)

It's great for IF.

• Applications: Immunofluorescence,
• Cell Tissue Type: Human

FH

Praveen (Verified Customer) (04-15-2019)

Good

• Applications: Western Blot,
• Cell Tissue Type: AC16

TOM20 Antibody Western Blot, validation ( dilution) in AC16 (Cat no:11802-1-AP)

FH

Daniela (Verified Customer) (03-19-2019)

A very specific and reliable antibody. For Western Blot, it identifies the correct MW band in cellular and mitochondrial lysate from either murine (N2a) and human (HeLa and U2OS) cell. For immunofluorescence, it is ideal for localization studies: it works perfectly to label mitochondria instead of plasmidic markers.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: Western Blot 1:2000, Immunofluorescence 1:200
• Cell Tissue Type: N2a, U2OS and HeLa cells

FH

Svitlana (Verified Customer) (03-19-2019)

SDS-PAGE: 15 ug protein/well.Lines on WB1. BioRad Precision Plus Protein standard2. Homogenate of mice brain tissue3. Non-synaptosomal mitochondria from mice brain.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hSecondary Ab:IRDye 680LT Goat anti-Rabbit, 1h incubation at room temperature.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mice brain tissue

TOM20 Antibody Western Blot, validation (1:2000 dilution) in Mice brain tissue (Cat no:11802-1-AP)

FH

Ken (Verified Customer) (02-06-2019)

It is an amazing antibody. Very specific and very bright at a 1:1000 dilution.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS cells

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