TOM70 Polyclonal antibody

• Featured Product
• KD/KO Validated

TOM70 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

78

Reviews (2)

Cat no : 14528-1-AP

Synonyms

TOMM70A, Translocase of outer mitochondrial membrane protein 70, TOMM70, TOMM, KIAA0719

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Validation Data Gallery

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  WB analysis of MCF-7 using 14528-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14528-1-AP (TOM70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14528-1-AP

  WB result of TOM70 antibody (14528-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOM70 transfected HeLa cells.

• 

  WB analysis of rat heart using 14528-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 14528-1-AP (TOM70 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 14528-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14528-1-AP (TOM70 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14528-1-AP

  IP result of anti-TOM70 (IP:14528-1-AP, 3ug; Detection:14528-1-AP 1:500) with mouse brain tissue lysate 6000ug.

• 

  IHC staining of human heart using 14528-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14528-1-AP (TOM70 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14528-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14528-1-AP (TOM70 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14528-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14528-1-AP (TOM70 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14528-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14528-1-AP (TOM70 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 14528-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14528-1-AP (TOM70 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 14528-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14528-1-AP (TOM70 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 14528-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14528-1-AP (TOM70 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 14528-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14528-1-AP (TOM70 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14528-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM70 antibody (14528-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 14528-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TOM70 Polyclonal antibody (14528-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, rat heart tissue, rat skeletal muscle tissue, mouse heart tissue, mouse brain tissue, mouse liver tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human heart tissue, human kidney tissue, mouse heart tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 65 publications below
• IHC: See 1 publications below
• IF: See 13 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

14528-1-AP targets TOM70 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TOM70 fusion protein Ag6027
• Full Name: translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC052994
• Gene Symbol: TOM70
• Gene ID (NCBI): 9868
• RRID: AB_2303727
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOMM70A (or TOM70) is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.

Publications

Species	Application	Title
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
mouse	WB	Nature; The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.; Authors - Atsushi Hoshino
human	WB	Nature; In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer.; Authors - Milica Momcilovic
human	WB	Nature; Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.; Authors - Sarraf Shireen A SA
human	WB	Nature; The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy.; Authors - Baris Bingol
	WB	Nat Biotechnol; Efficient proximity labeling in living cells and organisms with TurboID.; Authors - Tess C Branon

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (04-15-2021)

Cells firxed with 3.7% paraformaldehyde. Antibody visualized using anti-rabbit Alexa Fluor 647 secondary.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Cos7

TOM70 Antibody Immunofluorescence validation (1:100 dilution) in Cos7 (Cat no:14528-1-AP)

FH

Daniela (Verified Customer) (09-10-2019)

Used for Western Blot on total cell lysate from HeLa, this antibody identifies a strong band slightly below 75 kDa (proteins run on 15% acrylamide gel) and several fainter no-specific bands.Used for co-immunoprecipitation to detect over-expressed Flag-tagged protein interacting with Tom70, it identifies a sharp single band at the expected MW.

• Applications: Western Blot, Immunoprecipitation, Cell culture,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells

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