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TOM70 Polyclonal antibody

TOM70 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14528-1-AP

Synonyms

TOM70, TOMM70A



Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, rat heart tissue, rat skeletal muscle tissue, mouse heart tissue, mouse brain tissue, mouse liver tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman heart tissue, human kidney tissue, mouse heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

14528-1-AP targets TOM70 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOM70 fusion protein Ag6027
Full Name translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
Calculated molecular weight 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC052994
Gene symbol TOMM70A
Gene ID (NCBI) 9868
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOMM70A (or TOM70) is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.

Protocols

Product Specific Protocols
WB protocol for TOM70 antibody 14528-1-APDownload protocol
IHC protocol for TOM70 antibody 14528-1-APDownload protocol
IF protocol for TOM70 antibody 14528-1-APDownload protocol
IP protocol for TOM70 antibody 14528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.

Authors - Atsushi Hoshino
humanWB

Nature

In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer.

Authors - Milica Momcilovic
humanWB

Nature

Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.

Authors - Sarraf Shireen A SA
humanWB

Nature

The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy.

Authors - Baris Bingol
WB

Nat Biotechnol

Efficient proximity labeling in living cells and organisms with TurboID.

Authors - Tess C Branon
humanWB

Nat Methods

Directed evolution of APEX2 for electron microscopy and proximity labeling.

Authors - Stephanie S Lam

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (04-15-2021)

Cells firxed with 3.7% paraformaldehyde. Antibody visualized using anti-rabbit Alexa Fluor 647 secondary.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Cos7
TOM70 Antibody Immunofluorescence validation (1:100 dilution) in Cos7 (Cat no:14528-1-AP)
FH

Daniela (Verified Customer) (09-10-2019)

Used for Western Blot on total cell lysate from HeLa, this antibody identifies a strong band slightly below 75 kDa (proteins run on 15% acrylamide gel) and several fainter no-specific bands.Used for co-immunoprecipitation to detect over-expressed Flag-tagged protein interacting with Tom70, it identifies a sharp single band at the expected MW.

  • Applications: Western Blot, Immunoprecipitation, Cell culture,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cells