COX-1/Cyclooxygenase-1 Polyclonal antibody

• Featured Product
• KD/KO Validated

COX-1/Cyclooxygenase-1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

26

Reviews (1)

Cat no : 13393-1-AP

Synonyms

PGH1, PTGS1, Prostaglandin G/H synthase 1, PGHS-1, EC:1.14.99.1

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Validation Data Gallery

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  WB analysis of A431 using 13393-1-AP

  WB result of COX-1/Cyclooxygenase-1 antibody (13393-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-COX-1/Cyclooxygenase-1 transfected A431 cells.

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  WB analysis of HeLa using 13393-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13393-1-AP (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13393-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13393-1-AP (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 13393-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 13393-1-AP (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 13393-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 13393-1-AP (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13393-1-AP

  IP result of anti-COX-1/Cyclooxygenase-1 (IP:13393-1-AP, 4ug; Detection:13393-1-AP 1:500) with HeLa cells lysate 1280 ug.

• 

  IHC staining of human stomach using 13393-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13393-1-AP (COX-1/Cyclooxygenase-1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 13393-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13393-1-AP (COX-1/Cyclooxygenase-1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human stomach using 13393-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13393-1-AP (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, C2C12 cells, Neuro-2a cells, A431 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 26 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

13393-1-AP targets COX-1/Cyclooxygenase-1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COX-1/Cyclooxygenase-1 fusion protein Ag4055
• Full Name: prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
• Calculated Molecular Weight: 599 aa, 69 kDa
• Observed Molecular Weight: 60-72 kDa
• GenBank Accession Number: BC029840
• Gene Symbol: PTGS1
• Gene ID (NCBI): 5742
• RRID: AB_10644323
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTGS1(Prostaglandin G/H synthase 1) belongs to the prostaglandin G/H synthase family and catalyzes the conversion of arachidonic acid to prostaglandin H2, which is subsequently metabolized to various biologically active prostaglandins. It is also named as COX1(Cyclooxygenase-1). There is no PTGS1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues (PMID: 10999846). PTGS1 has some isoforms with MW of 56-72 kD and PTGS1 can form homodimer.

Publications

Species	Application	Title
rat	WB	Acta Pharm Sin B; Organic carbon monoxide prodrug, BW-CO-111, in protection against chemically-induced gastric mucosal damage.; Authors - Dominik Bakalarz
	WB	Small; M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation; Authors - Jie Sun
mouse	WB	Theranostics; Nanozyme-mediated catalytic nanotherapy for inflammatory bowel disease.; Authors - Jiulong Zhao
rat	WB	Elife; Hepatic AMPK signaling dynamic activation in response to REDOX balance are sentinel biomarkers of exercise and antioxidant intervention to improve blood glucose control; Authors - Meiling Wu
human	WB	Free Radic Biol Med; PET117 assembly factor stabilizes translation activator TACO1 thereby upregulates mitochondria-encoded cytochrome C oxidase 1 synthesis; Authors - Qiong Sun
mouse	WB	Ecotoxicol Environ Saf; Exposure to nicotine regulates prostaglandin E2 secretion and autophagy of granulosa cells to retard follicular maturation in mammals; Authors - Xiaofeng Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Thomas (Verified Customer) (09-18-2020)

HeLa cells were fixed in 4% PFA for 15 mins and permeabilised in 0.1% Triton-X 100 in PBS. Cells were blocked in 1% BSA. Primary antibody solution was diluted at 1:200 in blocking solution and incubated for 1 hour. Goat anti-rabbit 488 Alexa Fluor secondary antibody (1:250 - green) and DAPI (1:2000 - blue) were incubated with cells for 1 hour.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: HeLa

COX-1/Cyclooxygenase-1 Antibody Immunofluorescence validation (1:200 dilution) in HeLa (Cat no:13393-1-AP)