CD71 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CD71 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (4)

Anwendung

WB, IHC, IF/ICC, IP, ELISA, Cell treatment

Konjugation

Unkonjugiert

Publikationen

94

Rezensionen (2)

Kat-Nr. : 10084-2-AP

Synonyme

TFRC, TFR1, TFR, T9, sTfR

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Galerie der Validierungsdaten

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  WB analysis using 10084-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10084-2-AP (CD71 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10084-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10084-2-AP (CD71 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10084-2-AP

  IP result of anti-CD71 (IP:10084-2-AP, 4ug; Detection:10084-2-AP 1:500) with HeLa cells lysate 2400ug.

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  IHC staining of human placenta using 10084-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10084-2-AP (CD71 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10084-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10084-2-AP (CD71 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of K-562 using 10084-2-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (10084-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of K-562 using 10084-2-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (10084-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 77 publications below
• IHC: See 19 publications below
• IF: See 7 publications below

Produktinformation

10084-2-AP bindet in WB, IHC, IF/ICC, IP, ELISA, Cell treatment CD71 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Ente, Hausschwein, Huhn
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD71 fusion protein Ag0123
• Vollständiger Name: transferrin receptor (p90, CD71)
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC001188
• Gene symbol: CD71
• Gene ID (NCBI): 7037
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Publikationen

Species	Application	Title
human	WB	Cell Res; The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.; Authors - Jia-Yuan Zhang
human	IHC	Acta Neuropathol; Seizure-mediated iron accumulation and dysregulated iron metabolism after status epilepticus and in temporal lobe epilepsy.; Authors - Till S Zimmer
	WB,IHC	Cell Death Differ; Ferroptotic stress facilitates smooth muscle cell dedifferentiation in arterial remodelling by disrupting mitochondrial homeostasis; Authors - Qing-Xin Ji
	WB,IF	J Nanobiotechnology; Glioma-targeted oxaliplatin/ferritin clathrate reversing the immunosuppressive microenvironment through hijacking Fe2+ and boosting Fenton reaction; Authors - Xue Li
	WB	Acta Biomater; Cyclosporine A-loaded apoferritin alleviates myocardial ischemia-reperfusion injury by simultaneously blocking ferroptosis and apoptosis of cardiomyocytes; Authors - Wenqiang Qian
human	IHC	Cancer Res; Intratumoral payload release influences the potency and bystander-killing effects of antibody-drug conjugates in preclinical models.; Authors - Fu Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nu (Verified Customer) (10-24-2023)

Although there is a band close to 100 kd, there are other even more stronger non-specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: postmortem human frontal cortex

CD71 Antibody Western Blot validation (1:1000 dilution) in postmortem human frontal cortex (Cat no:10084-2-AP)

FH

Sara (Verified Customer) (10-25-2020)

Really good signal in WB, it worked great, even though I loaded 1-2 µg of total cell lysate in some lanes. Not that good for IF, the signal was a bit fuzzy and all over the cell.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 WB / 1:100 IF
• Cell Tissue Type: B cells from mouse spleen

CD71 Antibody Western Blot,Immunofluorescence validation (1:1000 WB / 1:100 IF dilution) in B cells from mouse spleen (Cat no:10084-2-AP)

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