TFEB Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TFEB Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA, Wes Immunoassay

Konjugation

Unkonjugiert

Publikationen

177

Rezensionen (5)

Kat-Nr. : 13372-1-AP

Synonyme

Transcription factor EB, TCFEB, Class E basic helix-loop-helix protein 35, bHLHe35, AlphaTFEB

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Galerie der Validierungsdaten

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  WB analysis using 13372-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13372-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13372-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13372-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 13372-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13372-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13372-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13372-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 13372-1-AP

  IP result of anti-TFEB (IP:13372-1-AP, 4ug; Detection:13372-1-AP 1:10000) with Raji cells lysate 2800 ug.

• 

  IHC staining of human renal cell carcinoma using 13372-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 13372-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13372-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TFEB antibody (13372-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, C2C12-Zellen, HEK-293-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, Mausherzgewebe, NIH/3T3-Zellen, Raji-Zellen, Rattenherzgewebe, Rattenhirngewebe, RAW 264.7-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 27 publications below
• WB: See 148 publications below
• IHC: See 15 publications below
• IF: See 80 publications below
• IP: See 6 publications below
• CoIP: See 2 publications below

Produktinformation

13372-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA, Wes Immunoassay TFEB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte, Rind, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TFEB fusion protein Ag4194
• Vollständiger Name: transcription factor EB
• Berechnetes Molekulargewicht: 476 aa, 53 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC032448
• Gene symbol: TFEB
• Gene ID (NCBI): 7942
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calculated molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).

Publikationen

Species	Application	Title
mouse	WB	ACS Nano; A Self-Assembled α-Synuclein Nanoscavenger for Parkinson's Disease.; Authors - Jingyi Liu
mouse	IF	Acta Neuropathol; Wolframin is a novel regulator of tau pathology and neurodegeneration.; Authors - Shuo Chen
mouse	WB,IF,IP	Mol Cell; Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.; Authors - Zhenxing Zhang
mouse	WB,IF	J Hazard Mater; VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes; Authors - Tao Wang
human	WB	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang ; KD Validated
mouse	WB	Mol Psychiatry; TFEB regulates lysosomal exocytosis of tau and its loss of function exacerbates tau pathology and spreading.; Authors - Yin Xu ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Stanislas (Verified Customer) (08-05-2024)

WB on HeLa lysates using 15µg of protein and nitrocellulose membranes, blockage in TBST-5%Milk 1h, Incubation in 2%BSA with TFEB antibody (1:3000) overnight at 4°C. Worked on the first trial. IF on HeLa fixed in 4%PFA for 15min, blocked 1h in 1XPBS, 5%NGS, 0.3% Triton X-100, incubates with TFEB antibody (1:100) in 1XPBS, 1%BSA, 0.3% Triton X-100 overnight at 4°C. Worked on first trial.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HeLa cells

FH

Shunbin (Verified Customer) (07-17-2023)

bands look specific, but blurry and multiple.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: B cell lines

TFEB Antibody Western Blot validation (1:2000 dilution) in B cell lines (Cat no:13372-1-AP)

FH

Weihao (Verified Customer) (04-11-2022)

Good antibody for mouse BMDM! not for human MDM 1:500 for IF 1:2000 for WB

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: mouse BMDM

FH

Marina (Verified Customer) (11-22-2021)

2 bands visible, unclear which is the right one, although size is ok-ish for both. Both bands are quite weak.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500 in 1% skim milk in TBST
• Cell Tissue Type: primary mouse microglia

FH

Tom (Verified Customer) (02-19-2021)

HEK293 lysate (10ug) subjected to SDS-PAGE and transferred to nitrocellullose. Membrane blocked in 5% BSA for 1 hour prior to incubation with antibody (1:1000 in block) overnight at 4 degrees. Band detected using anti-rabbit HRP secondary.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

TFEB Antibody Western Blot validation (1:1000 dilution) in HEK293 (Cat no:13372-1-AP)

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