SPIN1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SPIN1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 12105-1-AP

Synonyme

Spindlin1, spindlin 1, SPIN 1, SPIN, Ovarian cancer-related protein

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Galerie der Validierungsdaten

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  WB analysis using 12105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12105-1-AP (SPIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12105-1-AP (SPIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12105-1-AP

  WB result of SPIN1 antibody (12105-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPIN1 transfected HeLa cells.

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  IP experiment of HeLa using 12105-1-AP

  IP result of anti-SPIN1 (IP:12105-1-AP, 3ug; Detection:12105-1-AP 1:800) with HeLa cells lysate 1840ug.

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  IF Staining of HEK-293 using 12105-1-AP

  Immunofluorescent analysis of HEK-293 cells using 12105-1-AP (SPIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, PC-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

12105-1-AP bindet in WB, IHC, IF/ICC, IP, ChIP, ELISA SPIN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPIN1 fusion protein Ag2750
• Vollständiger Name: spindlin 1
• Berechnetes Molekulargewicht: 262 aa, 30 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC013571
• Gene symbol: SPIN1
• Gene ID (NCBI): 10927
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPIN1 (Spindlin 1) is a histone methylation reader, which recognizes trimethylated histone H3 lysine 4 through the protein-protein interaction. SPIN1 was initially found in ovarian cancer via searching ovarian cancer-related genes . It is overexpressed in ovarian cancer tissue, which exhibits a high homology to mouse Spin (Spindlin) gene. Signaling pathways responsible for SPIN1 functions include PI3K/Akt, Wnt and RET that are highly relevant to tumorigenesis.(PMID: 34492335, PMID: 31790762)

Publikationen

Species	Application	Title
human	WB	Nat Commun; Structural mechanism of bivalent histone H3K4me3K9me3 recognition by the Spindlin1/C11orf84 complex in rRNA transcription activation.; Authors - Yongming Du ; KD Validated
human	WB	Elife; SPIN1 promotes tumorigenesis by blocking the uL18 (universal large ribosomal subunit protein 18)-MDM2-p53 pathway in human cancer.; Authors - Ziling Fang ; KD Validated
human	WB, IP	PLoS Pathog; The Tudor Domain Protein Spindlin1 Is Involved in Intrinsic Antiviral Defense against Incoming Hepatitis B Virus and Herpes Simplex Virus Type 1.; Authors - Aurélie Ducroux ; KD Validated
human	WB,IF	EMBO Rep; Nucleolar protein Spindlin1 recognizes H3K4 methylation and stimulates the expression of rRNA genes.; Authors - Wang Weixiang W ; KD Validated
human	WB,IHC	Mol Oncol; E2F1-activated SPIN1 promotes tumor growth via a MDM2-p21-E2F1 feedback loop in gastric cancer.; Authors - Bei-Bei Lv ; KD Validated
human	WB	ACS Biomater Sci Eng; Soft Substrate Promotes Osteosarcoma Cell Self-Renewal, Differentiation, and Drug Resistance Through miR-29b and Its Target Protein Spin 1.; Authors - Shun Li

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