SULT2B1 Polyclonal antibody

SULT2B1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12879-1-AP

Synonyms

Sulfotransferase family 2B member 1, ST2B1, HSST2, EC:2.8.2.2, ARCI14

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Validation Data Gallery

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  WB analysis of mouse skin using 12879-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 12879-1-AP (SULT2B1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skin using 12879-1-AP

  IP result of anti-SULT2B1 (IP:12879-1-AP, 4ug; Detection:12879-1-AP 1:400) with mouse skin tissue lysate 3200ug.

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  IHC staining of human prostate cancer using 12879-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 12879-1-AP (SULT2B1 Antibody) at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human prostate cancer using 12879-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 12879-1-AP (SULT2B1 Antibody) at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human placenta using 12879-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 12879-1-AP (SULT2B1 Antibody) at dilution of 1:50.

• 

  IHC staining of human placenta using 12879-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 12879-1-AP (SULT2B1 Antibody) at dilution of 1:50.

• 

  IHC staining of human prostate cancer using 12879-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12879-1-AP (SULT2B1 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse skin tissue
• Positive IP detected in: mouse skin tissue
• Positive IHC detected in: human prostate cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12879-1-AP targets SULT2B1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SULT2B1 fusion protein Ag3838
• Full Name: sulfotransferase family, cytosolic, 2B, member 1
• Calculated Molecular Weight: 365 aa, 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC034694
• Gene Symbol: SULT2B1
• Gene ID (NCBI): 6820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SULT2B1 is a member of hydroxysteroid sulfotransferase (SULT) family that catalyzes sulfonation of hormones and other molecules. SULT2B1 encodes two isoforms, SULT2B1a and SULT2B1b, that differ only at their amino termini but have distinct biochemical properties as well as tissue distribution. The human fetal brain appears to only express the SULT2B1a isoform, while SULT2B1b is the predominant hydroxysteroid sulfotransferase expressed in human skin. Northern blot analysis revealed SULT2B1a has a limited tissue distribution in liver, adrenal and stomach, whereas SULT2B1b can be detected in a variety of hormone-responsive tissues including placenta, ovary, uterus, and prostate. This antibody recognizes both of SULT2B1a and SULT2B1b.

Publications

Species	Application	Title
human	WB	J Lipid Res; Activation of liver X receptor plays a central role in antiviral actions of 25-hydroxycholesterol.; Authors - Ying Liu
mouse	WB	Drug Metab Dispos; Sulfotransferase 4A1 (SULT4A1) increases its expression in mouse neurons as they mature.; Authors - Takuyu Hashiguchi