HDAC3 Polyclonal antibody

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  WB analysis of U-251 using 10255-1-AP

  WB result of HDAC3 antibody (10255-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDAC3 transfected U-251 cells.

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  WB analysis using 10255-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10255-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 10255-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 10255-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10255-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10255-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10255-1-AP (HDAC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 10255-1-AP

  IP result of anti-HDAC3 (IP:10255-1-AP, 3ug; Detection:10255-1-AP 1:300) with A431 cells lysate 1200ug.

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  IHC staining of human ovary tumor using 10255-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10255-1-AP (HDAC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 10255-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10255-1-AP (HDAC3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of K-562 using 10255-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using HDAC3 antibody (10255-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

HDAC3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10255-1-AP

Publications(67)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

Protein deacylase HDAC3, Protein deacetylase HDAC3, Histone deacetylase 3, HDAC 3, EC:3.5.1.98

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Tested Applications

• Positive WB detected in: A431 cells, HL-60 cells, HeLa cells, U-251 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 56 publications below
• IHC: See 9 publications below
• IF: See 7 publications below
• IP: See 4 publications below
• CoIP: See 3 publications below
• ChIP: See 2 publications below

Product Information

10255-1-AP targets HDAC3 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HDAC3 fusion protein Ag0396
• Full Name: histone deacetylase 3
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC000614
• Gene Symbol: HDAC3
• Gene ID (NCBI): 8841
• RRID: AB_2279733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15379
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the molecular weight of HDAC3? Is HDAC3 post-translationally modified?

The molecular weight of HDAC3 is 49 kDa but HDAC3 can form homodimers and homotrimers in cells (PMID: 11779848). HDAC3 can be phosphorylated at S424 residue by protein kinase CK2, which regulates HDAC3 activity (PMID: 15805470).

 What is the subcellular localization of HDAC3?

Unlike other class I HDACs, HDAC3 actively shuttles between the nucleus and cytoplasm (PMID: 11779848). Additionally, HDAC3 can be present at the plasma membrane, where it associates with c-Src (PMID: 16532030).

 What is the tissue expression pattern of HDAC3?

Like other class I HDACs, HDAC3 is ubiquitously expressed (PMID: 9346952).

 What is the mechanism of HDAC3 in regulating gene expression?

Unlike other histone deacetylates, it exists in a complex with two proteins - nuclear receptor co-repressor (N-CoR) and silencing mediator for retinoid and thyroid receptors (SMRT) - and therefore acts as a mediator of the repression function of unliganded nuclear hormone receptors (PMID: 10809664). N-CoR and SMRT play an active role in the recruitment of HDAC3 to binding sites as well as directly regulating HDAC3 activity. Additionally, HDAC3 targets growth-regulated genes.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Acta Pharm Sin B; Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.; Authors - Xin Sun
mouse	WB	J Clin Invest; Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.; Authors - Shanshan Zhong
human	WB	Mol Ther; Genetic and Pharmacological Inhibition of GRPR Protects Against Acute Kidney Injury via Attenuating Renal Inflammation and Necroptosis; Authors - Chao Li
mouse	WB	J Exp Med; Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.; Authors - Zhe Chen
rat	WB	Cell Rep; Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex.; Authors - Tian Wang
human	WB,IF	Cell Death Dis; Phosphatase LHPP confers prostate cancer ferroptosis activation by modulating the AKT-SKP2-ACSL4 pathway; Authors - Guoqing Xie