SNAI1 Polyklonaler Antikörper

SNAI1 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 26183-1-AP

Synonyme

Protein sna, Protein snail homolog 1, SLUGH2, SNA, SNAH, SNAI1, snail homolog 1 (Drosophila), SNAIL1, Zinc finger protein SNAI1

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 26183-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26183-1-AP (SNAI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 26183-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 26183-1-AP (SNAI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 26183-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26183-1-AP (SNAI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 26183-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 26183-1-AP (SNAI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 26183-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 26183-1-AP (SNAI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 26183-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26183-1-AP (SNAI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 26183-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26183-1-AP (SNAI1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, NIH/3T3-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

26183-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA SNAI1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNAI1 fusion protein Ag24248
• Vollständiger Name: snail homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 264 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29-35 kDa
• GenBank-Zugangsnummer: BC012910
• Gene symbol: SNAI1
• Gene ID (NCBI): 6615
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SNAI1, a member of SNAI1 family of protein, participates in the epithelial to mesenchymal transition(EMT) and formation and maintenance of embryonic mesoderm. The snail family share a common structural, that a highly conserved C-terminal region containing a zinc finger transcription factor. SNAI1 interacts with other corepressor, such as Ajuba, PRMT5 and SIN3a or HDAC1 and 2, to repress the target gene. As the phosphorylation modification of SNAI1 protein, the range of molecular weight of SNAI1 is about 25-30 kDa (PMID: 22276203 ). Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.

Publikationen

Species	Application	Title
human	WB	Oxid Med Cell Longev; XPO1-Mediated EIF1AX Cytoplasmic Relocation Promotes Tumor Migration and Invasion in Endometrial Carcinoma; Authors - Yuhong Ye
human	WB	Aging (Albany NY); Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma.; Authors - Ya Peng
human	IHC	Front Cell Dev Biol; Targeting MALT1 Suppresses the Malignant Progression of Colorectal Cancer via miR-375/miR-365a-3p/NF-κB Axis.; Authors - Rui Qian
human	WB	Oncotarget; AEG-1/MTDH-activated autophagy enhances human malignant glioma susceptibility to TGF-β1-triggered epithelial-mesenchymal transition.; Authors - Meijuan Zou
human	WB	J Cell Mol Med; Autophagy promotes fibrosis and apoptosis in the peritoneum during long-term peritoneal dialysis.; Authors - Jingjing Wu
human	WB	Cancer Manag Res; AOC1 Contributes to Tumor Progression by Promoting the AKT and EMT Pathways in Gastric Cancer.; Authors - Fen Xu

Verwandte Produkte

E-cadherin Polyclonal antibody

20874-1-AP

SNAI2/SLUG Polyclonal antibody

12129-1-AP

TWIST1-specific Polyclonal antibody

25465-1-AP

ZEB2 Polyclonal antibody

14026-1-AP

TGF Beta 1 Polyclonal antibody

18978-1-AP

GSK3B Polyclonal antibody

15113-1-AP

EGFR-Specific Polyclonal antibody

18986-1-AP