ZEB2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZEB2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

67

Cat no : 14026-1-AP

Synonyms

KIAA0569, SIP 1, SIP1, Smad interacting protein 1, SMADIP1, ZEB2, ZFHX1B, ZFX1B

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Validation Data Gallery

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  WB analysis using 14026-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14026-1-AP (ZEB2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14026-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14026-1-AP (ZEB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14026-1-AP

  WB result of ZEB2 antibody (14026-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZEB2 transfected HepG2 cells.

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  IHC staining of human lung cancer using 14026-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14026-1-AP (ZEB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 14026-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 14026-1-AP (ZEB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14026-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14026-1-AP (ZEB2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, HeLa cells
• Positive IHC detected in: human gliomas tissue, human lung cancer tissue, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 60 publications below
• IHC: See 7 publications below
• IF: See 4 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Product Information

14026-1-AP targets ZEB2 in WB, IHC, IF, ChIP, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, chicken, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZEB2 fusion protein Ag5171
• Full Name: zinc finger E-box binding homeobox 2
• Calculated Molecular Weight: 136 kDa
• Observed Molecular Weight: 150-170 kDa
• GenBank Accession Number: BC060819
• Gene Symbol: ZEB2
• Gene ID (NCBI): 9839
• RRID: AB_2878001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger E-box-binding homeobox (ZEB2) is a key transcription factor that acts as a multifunctional regulator during nervous system development. ZEB2 contains two zinc finger domains and a homeodomain-like sequence and interacts with the TGF-ß superfamily signaling regulators, Smads, to regulate the expression of their downstream genes. ZEB2 is expressed in the developing neural tube, as well as in neural crest cells, the hippocampus and the cerebral cortex. ZEB2 has previously been implicated in EMT, cell-cycle progression, apoptosis and senescence. ZEB2 was overexpressed in bladder, ovarian, stomach, pancreatic and squamous cell carcinoma, in the intestinal subtype of stomach cancers, and at the invasive front of CRC where EMT is most prominen. ZEB2 also mediates cell-fate decision in neuronal, T cells and hematopoietic stem cells. The calculated molecular weight of ZEB2 is 136 kDa, but we find the 95 kDa band in some publication(PMID: 27659015 )

Publications

Species	Application	Title
mouse	IF	Nat Commun; Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice.; Authors - Hans I-Chen Harn
human	WB,IF	Cell Death Differ; Regulation of Semaphorin3A in the process of cutaneous wound healing.; Authors - Yang Zheng
human	WB	Cancer Lett; A novel antagonist of the CCL5/CCR5 axis suppresses the tumor growth and metastasis of triple-negative breast cancer by CCR5-YAP1 regulation; Authors - Ling Chen
human	WB	Oncotarget; Pygopus-2 promotes invasion and metastasis of hepatic carcinoma cell by decreasing E-cadherin expression.; Authors - Sheng Zhang
human	WB	Cell Death Dis; PIM1 mediates epithelial-mesenchymal transition by targeting Smads and c-Myc in the nucleus and potentiates clear-cell renal-cell carcinoma oncogenesis.; Authors - Bin Zhao
human	WB	J Gastroenterol; LncRNA n339260 functions in hepatocellular carcinoma progression via regulation of miRNA30e-5p/TP53INP1 expression.; Authors - Tieju Liu