SMN (Human-Specific) Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

SMN (Human-Specific) Monoklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

2C6D9

Kat-Nr. : 60154-1-Ig

Synonyme

C BCD541, Component of gems 1, Gemin 1, SMN, SMN (Human-Specific), SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein

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Galerie der Validierungsdaten

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  WB analysis of A375 using 60154-1-Ig

  A375, HEK-293, and Jurkat cells were subjected to SDS PAGE followed by western blot with 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 60154-1-Ig

  WB result of SMN (Human-Specific) antibody (60154-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN (Human-Specific) transfected HEK-293 cells.

• 

  WB analysis of HepG2 using 60154-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 60154-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 60154-1-Ig

  IP Result of anti-SMN (Human-Specific) (IP:60154-1-Ig, 4ug; Detection:60154-1-Ig 1:500) with HEK-293 cells lysate 2440ug.

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  IHC staining of human brain using 60154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 60154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 60154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 60154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 60154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 60154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 60154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 60154-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN (Human-Specific) antibody (60154-1-Ig, Clone: 2C6D9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of Jurkat using 60154-1-Ig

  1X10^6 Jurkat cells were stained with 0.20ug SMN (Human-Specific) antibody (60154-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HEK-293-Zellen, HepG2-Zellen, Raji-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Herzgewebe, humanes Lebergewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

60154-1-Ig bindet in WB, IP, IHC, IF, FC, ELISA SMN (Human-Specific) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMN (Human-Specific) fusion protein Ag14333
• Vollständiger Name: survival of motor neuron 2, centromeric
• Berechnetes Molekulargewicht: 282 aa, 30 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC000908
• Gene symbol: SMN2
• Gene ID (NCBI): 6607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The survival of motor neurons (SMN) genes are the disease genes of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The level of SMN protein correlates with phenotypic severity of SMA. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional, because a large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein. This antibody 60154-1-Ig is specific to human SMN2. It can't recognize mouse and rat SMN.

Publikationen

Species	Application	Title
human		Nat Chem Biol; SMN2 splice modulators enhance U1-pre-mRNA association and rescue SMA mice.; Authors - James Palacino
human	WB	Science; Base editing rescue of spinal muscular atrophy in cells and in mice; Authors - Mandana Arbab

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