SEC61G Polyklonaler Antikörper

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• KD/KO Validated

SEC61G Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 11147-2-AP

Synonyme

Sec61 gamma subunit, SEC61G, SSS1

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Galerie der Validierungsdaten

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  WB analysis using 11147-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11147-2-AP (SEC61G antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11147-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11147-2-AP (SEC61G antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DC2.4 using 11147-2-AP

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 11147-2-AP (SEC61G antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11147-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11147-2-AP (SEC61G antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: EC109-Zellen, DC2.4-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 14 publications below
• IHC: See 7 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

11147-2-AP bindet in WB, IP, IF, IHC, ELISA SEC61G und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEC61G fusion protein Ag1624
• Vollständiger Name: Sec61 gamma subunit
• Berechnetes Molekulargewicht: 7.7 kDa
• Beobachtetes Molekulargewicht: 7.7 kDa
• GenBank-Zugangsnummer: BC009480
• Gene symbol: SEC61G
• Gene ID (NCBI): 23480
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SEC61G is a subunit of the SEC61 complex, which also contains alpha (SEC61A) and beta (SEC61B) subunits. The SEC61 complex is the central component of the protein translocation apparatus on the endoplasmic reticulum membrane. The gene of SEC61G maps to chromosome 7p11.2, and encodes a 68-amino acid single-pass membrane protein. This antibody can recognize endogenous SEC61G that migrates at a molecular mass of ~8 kDa.

Publikationen

Species	Application	Title
mouse	WB	Immunity; The translocon protein sec61 mediates antigen transport from endosomes in the cytosol for cross-presentation to CD8(+) T cells.; Authors - Matthias Zehner ; KD Validated
mouse	WB	Proc Natl Acad Sci U S A; Ablation of cholesterol biosynthesis in neural stem cells increases their VEGF expression and angiogenesis but causes neuron apoptosis.; Authors - Saito Kanako K
human	WB	Proc Natl Acad Sci U S A; A scalable strategy for high-throughput GFP tagging of endogenous human proteins.; Authors - Manuel D Leonetti
human	WB	Cancer Res; Glioblastoma proto-oncogene SEC61gamma is required for tumor cell survival and response to endoplasmic reticulum stress.; Authors - Lu Zheming Z ; KD Validated
human	WB	Elife; An allosteric Sec61 inhibitor traps nascent transmembrane helices at the lateral gate.; Authors - Andrew L Mackinnon
human	IHC	J Pathol; Genome-wide analysis of DNA copy number alterations and gene expression in gastric cancer.; Authors - Tsukamoto Y Y

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