SEC61 Alpha Polyclonal antibody

SEC61 Alpha Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 24935-1-AP

Synonyms

Sec61 alpha 2, SEC61A, SEC61A2, SEC61alpha

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Validation Data Gallery

• 

  WB analysis of mouse brain using 24935-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24935-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of U2OS using 24935-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61 Alpha antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24935-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IF Staining of HepG2 using 24935-1-AP

  Immunofluorescent analysis of HepG2 cells using 24935-1-AP (SEC61alpha antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293 cells, U2OS cells, mouse liver tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 24935-1-AP is SEC61 Alpha Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEC61 Alpha fusion protein Ag20779
• Full Name: Sec61 alpha 2 subunit (S. cerevisiae)
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC026179
• Gene Symbol: SEC61 Alpha
• Gene ID (NCBI): 55176
• RRID: AB_2879807
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The SEC61 complex is a major component of the endoplasmic reticulum (ER) translocation site (PMID: 7720858). This complex is composed of three subunits: SEC61alpha, SEC61beta and SEC61gamma. SEC61alpha is a polytopic integral membrane protein. It is a core component of the ER translocation complex, playing a crucial role in the insertion of secretory and membrane polypeptides into the ER (PMID: 9512475).

Publications

Species	Application	Title
human	IF	Sci Adv; Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.; Authors - Yuan Wang
rat	CoIP	iScience; The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors; Authors - Angela L. Whittsette

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (12-16-2021)

This antibody recognized a band around 60 kd, but it also recognized a similar band in solo loading buffer.

• Applications: Western Blot

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