SEC61B Polyclonal antibody

SEC61B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

19

Reviews (2)

Cat no : 15087-1-AP

Synonyms

Sec61 beta subunit, SEC61B

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Validation Data Gallery

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  WB analysis using 15087-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15087-1-AP (SEC61B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15087-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15087-1-AP (SEC61B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15087-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15087-1-AP (SEC61B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15087-1-AP

  IP result of anti-SEC61B (IP:15087-1-AP, 4ug; Detection:15087-1-AP 1:500) with HepG2 cells lysate 1800ug.

• 

  IHC staining of human liver using 15087-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15087-1-AP (SEC61B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 15087-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15087-1-AP (SEC61B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 15087-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15087-1-AP (SEC61B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 15087-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15087-1-AP (SEC61B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 15087-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (15087-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse pancreas using 15087-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (15087-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 15087-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SEC61B antibody (15087-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 15087-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15087-1-AP (SEC61B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, HeLa cells, mouse pancreas tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

15087-1-AP targets SEC61B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEC61B fusion protein Ag7169
• Full Name: Sec61 beta subunit
• Calculated Molecular Weight: 10 kDa
• Observed Molecular Weight: 10-15 kDa
• GenBank Accession Number: BC001734
• Gene Symbol: SEC61B
• Gene ID (NCBI): 10952
• RRID: AB_2186411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).

Publications

Species	Application	Title
human	WB	Science; QRICH1 dictates the outcome of ER stress through transcriptional control of proteostasis.; Authors - Kwontae You
mouse	WB	Nat Neurosci; Faulty autolysosome acidification in Alzheimer's disease mouse models induces autophagic build-up of Aβ in neurons, yielding senile plaques.; Authors - Ju-Hyun Lee
human	WB	Sci Adv; CReP mediates selective translation initiation at the endoplasmic reticulum.; Authors - Jonathan P Kastan
human	IF	J Cell Biol; Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway.; Authors - Satoshi Ninagawa
human	IF	Genes Dev; Identification of a localized nonsense-mediated decay pathway at the endoplasmic reticulum.; Authors - Dasa Longman
human,mouse	WB	Mol Ther; Efficient Gene Silencing by Adenine Base Editor-Mediated Start Codon Mutation.; Authors - Xinjie Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wei (Verified Customer) (09-22-2020)

SEC61B Antibody have correct localization on ER.

• Applications: Immunofluorescence
• Cell Tissue Type: Mouse heart

FH

WEI (Verified Customer) (06-29-2020)

Strong band for heart tissues, but not so sharp band.

• Applications: Western Blot,
• Cell Tissue Type: Mouse heart

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