SEC61B Polyclonal antibody
SEC61B Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15087-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human liver tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells, HeLa cells, mouse pancreas tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 11 publications below |
IHC | See 3 publications below |
IF | See 5 publications below |
IP | See 1 publications below |
Product Information
15087-1-AP targets SEC61B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, monkey, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SEC61B fusion protein Ag7169 |
Full Name | Sec61 beta subunit |
Calculated molecular weight | 10 kDa |
Observed molecular weight | 10-15 kDa |
GenBank accession number | BC001734 |
Gene symbol | SEC61B |
Gene ID (NCBI) | 10952 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).
Protocols
Product Specific Protocols | |
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WB protocol for SEC61B antibody 15087-1-AP | Download protocol |
IHC protocol for SEC61B antibody 15087-1-AP | Download protocol |
IF protocol for SEC61B antibody 15087-1-AP | Download protocol |
IP protocol for SEC61B antibody 15087-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Science QRICH1 dictates the outcome of ER stress through transcriptional control of proteostasis. | ||
Nat Neurosci Faulty autolysosome acidification in Alzheimer's disease mouse models induces autophagic build-up of Aβ in neurons, yielding senile plaques. | ||
J Cell Biol Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway. | ||
Genes Dev Identification of a localized nonsense-mediated decay pathway at the endoplasmic reticulum. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Wei (Verified Customer) (09-22-2020) | SEC61B Antibody have correct localization on ER.
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FH WEI (Verified Customer) (06-29-2020) | Strong band for heart tissues, but not so sharp band.
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