SEC61B Polyclonal antibody

SEC61B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 51020-2-AP


Sec61 beta subunit, SEC61B

Tested Applications

Positive WB detected inA431 cells, BxPC-3 cells, HEK-293T cells, HeLa cells, HepG2 cells, 3T3-L1 cells, C2C12 cells, RAW 264.7 cells, C6 cells
Positive IHC detected inmouse pancreas tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse pancreas tissue, HepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

51020-2-AP targets SEC61B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Sec61 beta subunit
Calculated molecular weight 10 kDa
Observed molecular weight 10-15 kDa
GenBank accession numberBC001734
Gene symbol SEC61B
Gene ID (NCBI) 10952
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).


Product Specific Protocols
WB protocol for SEC61B antibody 51020-2-APDownload protocol
IHC protocol for SEC61B antibody 51020-2-APDownload protocol
IF protocol for SEC61B antibody 51020-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Exp Med

Host ER-parasitophorous vacuole interaction provides a route of entry for antigen cross-presentation in Toxoplasma gondii-infected dendritic cells.

Authors - Goldszmid Romina S RS

Sci Rep

Neuroprotection mediated by cystatin C-loaded extracellular vesicles.

Authors - Rocío Pérez-González

Methods Mol Biol

Assessing Rab5 Activation in Health and Disease

Authors - Anna Pensalfini

Nat Commun

The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition

Authors - Rakesh Ganji