Validation Data Gallery
|Positive WB detected in||A431 cells, BxPC-3 cells, HEK-293T cells, HeLa cells, HepG2 cells, 3T3-L1 cells, C2C12 cells, RAW 264.7 cells, C6 cells|
|Positive IHC detected in||mouse pancreas tissue, mouse liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse pancreas tissue, HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunohistochemistry (IHC)||IHC : 1:300-1:1200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
51020-2-AP targets SEC61B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||Sec61 beta subunit|
|Calculated molecular weight||10 kDa|
|Observed molecular weight||10-15 kDa|
|GenBank accession number||BC001734|
|Gene ID (NCBI)||10952|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).
J Exp Med
Host ER-parasitophorous vacuole interaction provides a route of entry for antigen cross-presentation in Toxoplasma gondii-infected dendritic cells.
Neuroprotection mediated by cystatin C-loaded extracellular vesicles.
Methods Mol Biol
Assessing Rab5 Activation in Health and Disease
The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition