SEC61B-Specific Polyclonal antibody

SEC61B-Specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14846-1-AP


Sec61 beta subunit, SEC61B, SEC61B-Specific

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, human kidney tissue, mouse kidney tissue, mouse liver tissue, rat kidney tissue
Positive IHC detected inmouse liver tissue, human cervical cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse pancreas tissue, HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

14846-1-AP targets SEC61B-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Sec61 beta subunit
Calculated molecular weight 10 kDa
Observed molecular weight 10-15 kDa
GenBank accession numberNM_006808
Gene symbol SEC61B
Gene ID (NCBI) 10952
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).


Product Specific Protocols
WB protocol for SEC61B-Specific antibody 14846-1-APDownload protocol
IHC protocol for SEC61B-Specific antibody 14846-1-APDownload protocol
IF protocol for SEC61B-Specific antibody 14846-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



PLoS Pathog

Legionella hijacks the host Golgi-to-ER retrograde pathway for the association of Legionella-containing vacuole with the ER.

Authors - Mio Kawabata