P16-INK4A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

P16-INK4A Polyklonal Antikörper für WB, IHC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, hund und mehr (3)

Anwendung

WB, IHC, FC (Intra), IP, ELISA, Cell treatment

Konjugation

Unkonjugiert

Publikationen

346

Rezensionen (6)

Kat-Nr. : 10883-1-AP

Synonyme

CDKN2A, MTS1, CDKN2, CDK4I, CDK4 inhibitor p16INK4

---

Galerie der Validierungsdaten

• 

  WB analysis using 10883-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10883-1-AP (P16-INK4A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10883-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10883-1-AP (P16-INK4A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK293 cells using 10883-1-AP

  WB result of p16-INK4A antibody (10883-1-AP, 1:2,000) with si-Control and si-p16 transfected HEK-293 cells.

• 

  IP experiment of HEK-293 using 10883-1-AP

  IP result of anti-P16-INK4A (IP:10883-1-AP, 4ug; Detection:10883-1-AP 1:4000) with HEK-293 cells lysate 1400 ug.

• 

  IHC staining of human cervical cancer using 10883-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10883-1-AP (P16-INK4A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10883-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10883-1-AP (P16-INK4A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MDCK using 10883-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using P16-INK4A antibody (10883-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 10883-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human P16-INK4A (10883-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, PC-3-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MDCK-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 282 publications below
• IHC: See 2 publications below
• IP: See 1 publications below
• FC: See 2 publications below

Produktinformation

10883-1-AP bindet in WB, IHC, FC (Intra), IP, ELISA, Cell treatment P16-INK4A und zeigt Reaktivität mit human, hund

• Getestete Reaktivität: human, hund
• In Publikationen genannte Reaktivität: human, Affe, Hund, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P16-INK4A fusion protein Ag1328
• Vollständiger Name: cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 16-18 kDa
• GenBank-Zugangsnummer: BC021998
• Gene symbol: CDKN2A
• Gene ID (NCBI): 1029
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Background

p16 is an important cell cycle regulator and acts as a tumor suppressor. It may also be referred to as one of a number of synonyms, including p16INK4a and cyclin-dependent kinase inhibitor 2A.

 What is the molecular weight of P16?

16kDa. P16 is encoded by the CDKN2A gene in humans and is a chain comprising 148 amino acids.

What is the function of p16?

P16 inhibits cells from progressing from G1 into S phase, binding to cyclin-dependent kinase 4 (CDK4) and inhibiting its kinase ability, so that it cannot phosphorylate the retinoblastoma tumor suppressor (RB). Without this phosphorylation, RB does not activate downstream genes, so the G1/S checkpoint cannot be passed and the cell does not proliferate (PMID: 8259215).

 What is the role of p16 in senescence?

In senescence, cells are irreversibly arrested in the cell cycle. P16 is expressed more highly in aging tissue, is associated with intrinsic cellular aging signals such as telomere shortening, and can therefore be used as a marker of senescence (PMID: 9244355; PMID: 19535234). Due to its role in cell cycle arrest, p16 drives the initiation and maintenance of a cellular senescent phenotype.

 What is the role of p16 in cancer?

As a negative regulator of proliferation, p16 is a known tumor suppressor. Mutations in the CDKN2A gene that lead to inactivation of p16 protein have been associated with an increased risk of cancer and are often observed in primary tumors and in cancer cell lines (PMID: 9508208). The inactivation of p16 has been shown to be a key early stage of tumor progression. In a small number of tumor types that are caused by the human papilloma virus (HPV), p16 is in fact overexpressed when RB is inactivated, releasing p16 and causing an accumulation (PMID: 21297668).

Publikationen

Species	Application	Title
		Nature; Senolytic CAR T cells reverse senescence-associated pathologies.; Authors - Corina Amor
human	WB	Science; PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation.; Authors - Federico Gulluni
human		Nat Genet; Early TP53 alterations engage environmental exposures to promote gastric premalignancy in an integrative mouse model.; Authors - Nilay S Sethi
	WB	Cancer Cell; mTORC1 Activation Blocks Braf(V600E)-Induced Growth Arrest but Is Insufficient for Melanoma Formation.; Authors - William Damsky
human	WB	Circulation; Vascular Smooth Muscle Cell Senescence Promotes Atherosclerosis and Features of Plaque Vulnerability.; Authors - Julie Wang
	WB	Cell Metab; SREBP1c-PARP1 axis tunes anti-senescence activity of adipocytes and ameliorates metabolic imbalance in obesity.; Authors - Gung Lee

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Vikas (Verified Customer) (04-23-2024)

Highly recommended, to use 1:1000 dilution for Western bloating.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y cell line

FH

Vikas (Verified Customer) (04-23-2024)

Used p16 antibody for western-bolting at the dilution 1:10000, antibody worked at best. Thank you, highly recommended it.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y cell line

FH

Susanne (Verified Customer) (10-11-2022)

blocking: ROTIblock Incubation over night 4°C

• Applications: Western Blot
• Primary Antibody Dilution: 1 : 1000
• Cell Tissue Type: mouse skin tissue

FH

Malak (Verified Customer) (04-22-2021)

Unfortunately, with this antibody, I didn't get a bad at 16 KD, however it was higher (around 20 KD). The suppliers were so professional and offered their help to optimize this antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Huh7 cells extract

FH

Joshua (Verified Customer) (03-12-2020)

MDCKs fixed in 4% paraformaldehyde and stained overnight at 4 C. Excellent, bright stain.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MDCK

P16-INK4A Antibody Immunofluorescence, validation (1:500 dilution) in MDCK (Cat no:10883-1-AP)

FH

Kyle (Verified Customer) (02-28-2019)

Clean bands, low background

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: small cell lung cancer

Verwandte Produkte

P53 Monoclonal antibody

60283-1-Ig

P21 Polyclonal antibody

10355-1-AP

RB1 Polyclonal antibody

10048-2-Ig

CDK4 Polyclonal antibody

11026-1-AP

CDK6 Polyclonal antibody

14052-1-AP

p15 INK4b Polyclonal antibody

12877-1-AP