CDK6 Polyclonal antibody

• Featured Product
• KD/KO Validated

CDK6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

187

Cat no : 14052-1-AP

Synonyms

Serine/threonine-protein kinase PLSTIRE, EC:2.7.11.22, Cyclin-dependent kinase 6, Cyclin dependent kinase 6, Cell division protein kinase 6

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Validation Data Gallery

• 

  WB analysis using 14052-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14052-1-AP (CDK6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14052-1-AP

  WB result of CDK6 antibody (14052-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK6 transfected HeLa cells.

• 

  WB analysis of MOLT-4 using 14052-1-AP

  MOLT-4 cells were subjected to SDS PAGE followed by western blot with 14052-1-AP (CDK6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14052-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14052-1-AP (CDK6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14052-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14052-1-AP (CDK6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 cells using 14052-1-AP

  C2C12 cells (shRNA samples) were subjected to SDS PAGE followed by western blot with 14052-1-AP (CDK6 antibody) at dilution of 1:500. .

• 

  IP experiment of Jurkat using 14052-1-AP

  IP result of anti-CDK6 (IP:14052-1-AP, 4ug; Detection:14052-1-AP 1:4000) with Jurkat cells lysate 1400 ug.

• 

  IHC staining of mouse colon using 14052-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 14052-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 14052-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 14052-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14052-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14052-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 14052-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 14052-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 14052-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14052-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK6 antibody (14052-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 14052-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK6 Polyclonal antibody (14052-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, C2C12 cells, HeLa cells, Jurkat cells, MOLT-4 cells, NIH/3T3 cells, K-562 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human lung cancer tissue, human endometrial cancer tissue, human urothelial carcinoma tissue, mouse colon tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 176 publications below
• IHC: See 20 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

14052-1-AP targets CDK6 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDK6 fusion protein Ag5275
• Full Name: cyclin-dependent kinase 6
• Calculated Molecular Weight: 326 aa, 36 kDa
• Observed Molecular Weight: 36-40 kDa
• GenBank Accession Number: BC027989
• Gene Symbol: CDK6
• Gene ID (NCBI): 1021
• RRID: AB_10642144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin-dependent kinase 6 (CDK6) is an enzyme belonging to the CDK family. CDK6 is a catalytic subunit of a 

protein kinase complex that is essential for progression through the G1 phase of the cell cycle and G1/S transition, 

thus activating cell proliferation. CDK6 partners with cyclins to phosphorylate the retinoblastoma (pRb) protein to 

release its binding partner E2F; a transcription factor that drives gene expression necessary for DNA replication 

(PMID: 8114739). CDK6 was long thought of as a redundant homolog of CDK4 as they perform similar roles in cell 

cycle progression. But recently it has become apparent that CDK6 and CDK4 differ in tissue-specific functions, 

where CDK6 plays a role in differentiation (PMID: 16410727) but also in contribution to tumor development.

What is the molecular weight of CDK6?

The molecular weight of CDK6 is approximately 36-40 kDa (PMID: 23563707, 8114739).

What is the subcellular localization of CDK6?

CDK6 is ubiquitously expressed through the nucleus and cytoplasm. However, the most active complexes are 

located in the nuclei of proliferating cells.

What is the expression pattern of CDK6?

CDK6 activity first occurs midway through the G1 phase. Its expression is regulated by D-type cyclins and 

members of the INK4 family of CDK inhibitors (PMID: 7739547).

What is CDK6's role in cancer?

CDK6 is involved in a critical regulatory point in the cell cycle and so is found to be unbalanced in approximately 

80-90% of tumors (PMID: 23356980). Loss of cell cycle control is the initial step in the development of the 

hallmarks of cancer, such as sustained proliferative signaling and dysregulated cellular energetics. Deregulation 

of CDK6 has an important role in lymphoma by increasing angiogenesis (PMID: 23948297) and upregulated CDK6 

expression is also associated with a poor clinical prognosis in medulloblastoma (PMID: 23172372).

Publications

Species	Application	Title
human	WB	Nat Cell Biol; miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.; Authors - Cao Jingli J
human	WB,IHC	Nat Commun; The RNA-binding protein LRPPRC promotes resistance to CDK4/6 inhibition in lung cancer; Authors - Wei Zhou
human	WB	Genome Biol; Single-cell transcriptomics reveals multiple chemoresistant properties in leukemic stem and progenitor cells in pediatric AML; Authors - Yongping Zhang
human	WB	Nat Commun; ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.; Authors - Rokana Taftaf
human	WB	Nat Commun; Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.; Authors - Dan Su
mouse	WB	Dev Cell; DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice; Authors - Dingfeng Zou

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