ORAI1 Polyklonaler Antikörper

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• KD/KO Validated

ORAI1 Polyklonal Antikörper für WB, IF, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

43

Kat-Nr. : 13130-1-AP

Synonyme

CRACM1, ORAI1 L1, ORAI1 L2, Orail L2, ORAT1, Protein orai 1, TMEM142A, Transmembrane protein 142A

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 13130-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 13130-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13130-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 13130-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 13130-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13130-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13130-1-AP (ORAI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 13130-1-AP

  Immunofluorescent analysis of HEK-293 cells using 13130-1-AP (ORAI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A375-Zellen, Maus-Eierstockgewebe, Mausmilzgewebe, PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 35 publications below
• IF: See 8 publications below

Produktinformation

13130-1-AP bindet in WB, IF, ELISA ORAI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ORAI1 fusion protein Ag3758
• Vollständiger Name: ORAI calcium release-activated calcium modulator 1
• Berechnetes Molekulargewicht: 301 aa, 33 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC015369
• Gene symbol: ORAI1
• Gene ID (NCBI): 84876
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.

Publikationen

Species	Application	Title
human	WB,IF	Cell Res; Direct control of store-operated calcium channels by ultrafast laser.; Authors - Pan Cheng ; KO Validated
mouse	WB	Blood; Orai1 (CRACM1) is the platelet SOC channel and essential for pathological thrombus formation.; Authors - Braun Attila A
mouse	WB	Blood; The serum- and glucocorticoid-inducible kinase 1 (SGK1) influences platelet calcium signaling and function by regulation of Orai1 expression in megakaryocytes.; Authors - Borst Oliver O
mouse	WB	Kidney Int; AMP-activated kinase is a regulator of fibroblast growth factor 23 production.; Authors - Philipp Glosse
mouse	IF	Sci Signal; Adhesion to laminin-1 and collagen IV induces the formation of Ca2+ microdomains that sensitize mouse T cells for activation; Authors - Mariella Weiß
mouse	IF	Sci Signal; ORAI1, STIM1/2, and RYR1 shape subsecond Ca2+ microdomains upon T cell activation.; Authors - Björn-Philipp Diercks

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