Calmodulin Polyclonal antibody

Calmodulin Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 10541-1-AP

Synonyms

Calmodulin 3, Calmodulin 1/2/3, CALML2, CALM3, CAM3

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Validation Data Gallery

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  WB analysis using 10541-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 10541-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10541-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10541-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10541-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10541-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Ramos using 10541-1-AP

  IP result of anti-Calmodulin (IP:10541-1-AP, 2ug; Detection:10541-1-AP 1:600) with Ramos cells lysate 1120 ug.

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  IHC staining of human breast cancer using 10541-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10541-1-AP (Calmodulin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10541-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10541-1-AP (Calmodulin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10541-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10541-1-AP (Calmodulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10541-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10541-1-AP (Calmodulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 10541-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Calmodulin (10541-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of MCF-7 using 10541-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Calmodulin (10541-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, HepG2 cells, mouse brain tissue, MCF-7 cells, HeLa cells, NCCIT cells, rat skeletal muscle tissue
• Positive IP detected in: Ramos cells
• Positive IHC detected in: human breast cancer tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IP: See 2 publications below

Product Information

10541-1-AP targets Calmodulin in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calmodulin fusion protein Ag0827
• Full Name: calmodulin 3 (phosphorylase kinase, delta)
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC006182
• Gene Symbol: Calmodulin 3
• Gene ID (NCBI): 808
• RRID: AB_2069442
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calmodulin (CaM) is a Ca(2+)-binding protein that transduces Ca2+-mediated signals by binding to and regulating the activity of hundreds of enzymes and non-enzymatic proteins. It is highly conserved across species and involved in many biological processes, including vesicle release, cell proliferation, and apoptosis. This antibody can recognize CALM1, CALM2 and CALM3.

Publications

Species	Application	Title
human	WB	Nat Commun; Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis; Authors - Ji Geng
human		Autophagy; Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy; Authors - Kui Wang
human	IHC, WB	Cancer Cell Int; CALM1 promotes progression and dampens chemosensitivity to EGFR inhibitor in esophageal squamous cell carcinoma.; Authors - Tao Liu
	IP	Neurochem Int; Alpha-synuclein overexpression increases phospho-protein phosphatase 2A levels via formation of calmodulin/Src complex.; Authors - W Yang
human	WB	Biochem Biophys Res Commun; The NMDAR GluN1-1a C-terminus binds to CaM and regulates synaptic function.; Authors - Liang Zhou
human	WB	J Cardiovasc Transl Res; TRPC1 Deficiency Impairs the Endothelial Progenitor Cell Function via Inhibition of Calmodulin/eNOS Pathway.; Authors - Lai-Ling Du