STIM1 Polyclonal antibody

• Featured Product
• KD/KO Validated

STIM1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

64

Reviews (2)

Cat no : 11565-1-AP

Synonyms

stromal interaction molecule 1, STRMK, stim-1, STIM 1, IMD10

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Validation Data Gallery

• 

  WB analysis using 11565-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11565-1-AP (STIM1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 11565-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11565-1-AP (STIM1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 11565-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11565-1-AP (STIM1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11565-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11565-1-AP (STIM1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 11565-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11565-1-AP (STIM1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HEK-293 using 11565-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using STIM1 antibody (11565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 11565-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human STIM1 (11565-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human ovary tumor tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 15 publications below
• WB: See 47 publications below
• IHC: See 8 publications below
• IF: See 20 publications below
• IP: See 3 publications below
• FC: See 3 publications below

Product Information

11565-1-AP targets STIM1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STIM1 fusion protein Ag2140
• Full Name: stromal interaction molecule 1
• Calculated Molecular Weight: 685 aa, 77 kDa
• Observed Molecular Weight: 77-90 kDa
• GenBank Accession Number: BC021300
• Gene Symbol: STIM1
• Gene ID (NCBI): 6786
• RRID: AB_2302808
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms. Catalog#11565-1-AP is rabbit polyclonal antibody raised against the N-terminal of human STIM1.

Publications

Species	Application	Title
mouse	WB	Cell Res; Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate.; Authors - Ao Hu ; KO Validated
human	FC,IF	J Immunother Cancer; PD1 blockade enhances K+ channel activity, Ca2+ signaling, and migratory ability in cytotoxic T lymphocytes of patients with head and neck cancer.; Authors - Hannah S Newton
mouse	WB, IF	Proc Natl Acad Sci U S A; STIM1-Ca2+ signaling modulates automaticity of the mouse sinoatrial node.; Authors - Hengtao Zhang
human,mouse	WB	Cell Mol Life Sci; STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons; Authors - Karolina Serwach ; KD Validated
human	WB, IF	Int J Mol Sci; Expression and Localization of Thrombospondins, Plastin 3, and STIM1 in Different Cartilage Compartments of the Osteoarthritic Varus Knee.; Authors - Daniela Mählich
human	WB,IF	Oncogenesis; Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth; Authors - Chunming Gu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-16-2023)

Worked well in WB at 1:1000

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma cells

FH

lieqi (Verified Customer) (12-19-2018)

• Applications: Western Blot,
• Cell Tissue Type: T84

STIM1 Antibody Western Blot, validation ( dilution) in T84 (Cat no:11565-1-AP)

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