OIP5 Polyklonaler Antikörper

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• KD/KO Validated

OIP5 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 12142-1-AP

Synonyme

5730547N13Rik, Cancer/testis antigen 86, CT86, LINT 25, MIS18B, MIS18beta, OIP 5, OIP5, Opa interacting protein 5, Protein Mis18 beta

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Galerie der Validierungsdaten

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  WB analysis using 12142-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12142-1-AP (OIP5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12142-1-AP

  WB result of OIP5 antibody (12142-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OIP5 transfected Jurkat cells.

• 

  WB analysis using 12142-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12142-1-AP (OIP5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12142-1-AP

  IP result of anti-OIP5 (IP:12142-1-AP, 4ug; Detection:12142-1-AP 1:1500) with Jurkat cells lysate 2030 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen
• Erfolgreiche IP: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Produktinformation

12142-1-AP bindet in WB, IP, IF, IHC, ELISA OIP5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OIP5 fusion protein Ag2790
• Vollständiger Name: Opa interacting protein 5
• Berechnetes Molekulargewicht: 229 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC015050
• Gene symbol: OIP5
• Gene ID (NCBI): 11339
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OIP5 is also known as Mis18β. Vertebrates possess two Mis18 paralogs, Mis18α and Mis18β. Mis18 has been previously shown to affect histone modifications and the methylation status of underlying chromatin . Eliminating histone methylation within the alpha satellite DNA repeats of a human artificial chromosome alters the recruitment of HJURP .The Mis18core complex was originally characterized as an oligomer with a proposed 2:2 stoichiometry of Mis18α and Mis18β, but additional work demonstrated a 4:2 Mis18α:Mis18β hexamer. (PMID: 31492860, PMID: 26942680)

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Sci Bull (Beijing); Engineering redirected NF-κB/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer; Authors - Binbin Zheng
human	WB	Neuro Oncol; Cancer-testis specific gene OIP5: a downstream gene of E2F1 that promotes tumorigenesis and metastasis in glioblastoma by stabilizing E2F1 signaling.; Authors - Jiang He ; KD Validated
human	WB	Oncotarget; OIP5, a target of miR-15b-5p, regulates hepatocellular carcinoma growth and metastasis through the AKT/mTORC1 and β-catenin signaling pathways.; Authors - Hua Li
human	WB	J Biol Chem; Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone HJURP.; Authors - Jianyu Wang ; KD Validated
human	WB,IHC,IF	Int J Oncol; Characterization of Opa interacting protein 5 as a new biomarker and therapeutic target for oral cancer.; Authors - Ming Zhu ; KD Validated
human	WB,IHC,IF	Cancer Sci; Characterization of an Opa interacting protein 5 involved in lung and esophageal carcinogenesis.; Authors - Koinuma Junkichi J

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